| 赵昉,郑绍琴.青蒿素衍生物对非小细胞肺癌A549细胞增殖的影响[J].浙江中西医结合杂志,2012,22(2):94-96 |
| 青蒿素衍生物对非小细胞肺癌A549细胞增殖的影响 |
| Inhibition effect of Artemisinin and Its Derivatives on A549 Cells |
| 投稿时间：2011-08-31 |
| DOI： |
| 中文关键词: 青蒿素衍生物 抗肿瘤 非小细胞肺癌 A549细胞 |
| 英文关键词:artemisinin derivatives anti-tumor non-small cell lung cancer A549 cells |
| 基金项目: |
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| 中文摘要: |
| 目的：观察青蒿素衍生物对非小细胞肺癌A549 增殖的影响。方法：通过MTT 法初步对青
蒿素、双氢青蒿素和青蒿琥酯进行TC50 的测定，选择低毒剂量TC10 作为实验浓度，再以动态MTT
法观察各青蒿素衍生物在低毒剂量下对非小细胞肺癌A549 增殖的影响。结果：青蒿素、双氢青蒿
素、青蒿琥酯的TC50 分别为1.77 mmol/L、58.24 μmol/L 、64.33 μmol/L； 在对A549 细胞增殖影响
中，双氢青蒿素和青蒿琥酯能延长细胞倍增时间，减缓细胞增殖周期，但青蒿素对细胞倍增时间没
有影响，仅能延长细胞到达高峰期的时间。结论：青蒿素衍生物中，青蒿素抗肿瘤作用稍弱，青蒿琥
酯和双氢青蒿素抗肿瘤作用较好，能同时延长细胞倍增时间和增殖周期，显示对肺癌A549 细胞的
增殖有直接抑制作用。 |
| 英文摘要: |
| Objective: To observe the inhibitory effect of artemisinin and its derivatives on non-small cell lung cancer
A549 cells. Methods: The TC50（concentration required to cause 50% toxicity compared with controls）of artemisinin, dihydroartemisinin,
and artesunate on A549 cells was detemined by MTT assay. The TC10 was selected as the experimental
dosage in the proliferation inhibition of A549 assay. Results: The TC50 of artemisinin, dihydroartemisinin, and artesunate
were 1.77 mmol/L,58.24 μmol/L,64.33 μmol/L, respectively. Artemisinin had no direct inhibition effect on the proliferation
of A549,but dihydroartemisinin and artesunate prolonged the the cell cycle progression by delaying the double proliferation
time. Conclusion: Dihydroartemisinin and artesunate may have direct inhibitory effect on the proliferation of A549 cells. |
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