| 江德富,童夏生,罗冬娇,亢晓冬,叶 辉,范广民,陈 琪.哮喘大鼠TLR1、 FasL和TRAF2表达及甲基强的松龙干预作用[J].浙江中西医结合杂志,2012,22(6):424-427 |
| 哮喘大鼠TLR1、 FasL和TRAF2表达及甲基强的松龙干预作用 |
| Regulation with Methylprednisolone on the Expression of Toll like receptor 1, FasL, and Tumor NecrosisFactor Receptor Associated Factor 2 in Asthmatic Rats |
| 投稿时间：2012-01-13 |
| DOI： |
| 中文关键词: 大鼠 哮喘 TLR1 FasL TRAF2 肿瘤坏死因子受体相关因子 糖皮质激素 |
| 英文关键词:rats asthma Toll like receptor FasL tumor necrosis factor receptor associated factor glucocorti?
coids |
| 基金项目:浙江省医药卫生科技计划项目 （No.2007-B238）， 浙江省温岭市科技局基金资助项目 （No.2009-2-55） |
|
| 摘要点击次数: 1459 |
| 全文下载次数: 18 |
| 中文摘要: |
| 目的： 探讨TLR1、 FasL 和TRAF2在大鼠哮喘炎症机制中的作用， 观察甲基强的松龙对其
表达的影响。方法： 27只SD大鼠， 随机分成哮喘模型组、 正常对照组和甲基强的松龙组， 每组9只，
采用流式细胞术检测血淋巴细胞TLR1和FasL表达， 免疫组织化学法检测肺组织TRAF2表达。结
果： 哮喘组血淋巴细胞TLR1表达水平显著低于甲基强的松龙组 （P<0.05）， 正常对照组血淋巴细胞
TLR1表达水平分别与哮喘组及甲基强的松龙组比较， 差异无统计学意义 （P均>0.05）。哮喘组和
甲基强的松龙组血淋巴细胞FasL表达水平均显著高于正常对照组 （P均<0.05）， 而哮喘组血淋巴细
胞FasL表达水平与甲基强的松龙组比较差异无统计学意义 （P>0.05）。哮喘组TRAF2光密度值显
著高于正常对照组和甲基强的松龙组 （P均<0.01）， 甲基强的松龙组TRAF2光密度值与正常对照组
比较， 差异无统计学意义 （P>0.05）。结论： 哮喘模型大鼠FasL和TRAF2表达增加， 而TLR1无变化；
甲基强的松龙能下调TRAF2和提升TLR1水平， 从而起到抗炎作用。 |
| 英文摘要: |
| Objective： To investigate the potential effects of methylprednisolone on the expression of Toll like re?
ceptor 1(TLR1), FasL, and tumor necrosis factor receptor associated factor 2(TRAF2) in asthmatic rats. Methods:
Twenty-seven SD rats were randomly divided into 3 groups, including asthma group, control group, and methyl?
prednisolone-treated group. The levels of TLR1 and FasL in blood lymphocyte were detected by flow cytometry.
The expression of TRAF2 protein was detected by immunohistochemical methods. Results: The level of TLR1 in
the asthma group（47.58 ± 1.46 ）MFI was significantly lower than that in the methylprednisolone-treated group
（52.89±3.92 ） MFI (P<0.05); The level of TLR1 in the control group did not differ to that in the asthma group
or the methylprednisolone-treated group（all P>0.05） . The level of FasL in the asthma group（15.52±0.53 MFI）
and the methylprednisolone-treated group （14.46±2.19 MFI）were both significantly higher than that in the con?
trol group （11.73±1.39 MFI） (all P<0.05), but no significant difference was noted between the asthma group and
the methylprednisolone-treated group(P>0.05). The expression of TRAF2 protein in the asthma group (0.317 ±
0.041 optical density) was dramatically higher than that in the control group （0.220±0.057 optical density） and the
methylprednisolone treated group(0.235 ± 0.028 optical density) （all P<0.01） , but no significant difference was
seen between the methylprednisolone-treated group and the control group（P>0.05） . Conclusion: The levels of
FasL and TRAF2 were elevated and TLR1 had no change in asthmatic rats. The anti-inflammation role of meth?
ylprednisolone may be partly through down-regulating TRAF2 and up-regulating TLR1, but had no effect on
FasL. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
