| 刘立宾,叶华丽,胡建成,何月龙,范大鹏.支气管肺泡灌洗液TB-DNA测定与
其它TB检测方法比较研究[J].浙江中西医结合杂志,2013,23(2):95-97 |
| 支气管肺泡灌洗液TB-DNA测定与
其它TB检测方法比较研究 |
| Relationship Between fluorescent quantitative-PCR Detecting Bronchoalveolar Lavage Fluid TuberculosisDNA and other Tuberculosis detecting methods |
| 投稿时间：2012-09-26 |
| DOI： |
| 中文关键词: 支气管肺泡灌洗液
结核杆菌
荧光定量PCR |
| 英文关键词:tuberculosis
bronchoalveolar lavage fluid
fluorescent quantitative PCR |
| 基金项目: |
|
| 摘要点击次数: 1554 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的：探讨支气管灌洗液涂片染色、支气管灌洗液培养及痰培养三种方法中结核杆菌阳
性率与支气管灌洗液结核杆菌DNA拷贝数的关系。方法：对199例确诊肺结核患者分别应用荧光
定量聚合酶链反应（FQ-PCR）技术检测支气管灌洗液（BALF）结核分枝杆菌DNA（TB-DNA），并按
TB-DNA拷贝数分为A（1×103～）、B（1×105～）、C（≥1×107）三组，并对所有患者行灌洗液涂片染色、
灌洗液培养及痰培养结核杆菌。结果：灌洗液涂片染色法 A、B、C 三组阳性率分别为 12.0%、
60.2%、92.7%，三组间阳性率比较差异有统计学意义（P<0.05），三组间两两比较差异有统计学意义
（P均<0.05），且阳性率C组＞B组＞A组；灌洗液培养法A、B、C三组阳性率分别为18.7%、56.6%、
87.8%，三组间差异有统计学意义（P<0.05），两两比较差异有统计学意义（P均<0.05），阳性率C组
＞B组＞A组；痰培养法A、B、C三组阳性率分别为26.7%、65.1%、82.9%，三组间比较差异有统计学
意义（P<0.05），两两比较差异有统计学意义，A组与B组、A组与C组比较P均<0.05，B组与C组比
较P<0.05。结论：支气管灌洗液涂片染色、灌洗液培养、痰培养结核杆菌阳性率均随支气管灌洗液
中结核杆菌DNA拷贝数递增而增高。 |
| 英文摘要: |
| Objective：To investigate the relationship between the copy number of tuberculosis-DNA(TB-DNA）in
bronchoalveolar lavage fluid (BALF） by fluorescent quantitative PCR (FQ-PCR) and the positive rates of BALF
smearing, BALF training,and sputum training. Methods: TB-DNA in BALF from 199 definite tuberculosis pa?
tients was determined by FQ-PCR and then all samples were randomly divided into group A(1×103~）, group B
（1×105~）,and group C（≥1×107）.All samples were treated with TB BALF smearing,TB BALF training, and TB
sputum training. Results: In TB BALF smearing, the positive rate was significantly different in group A,B and C
(12.0%,60.2% and 92.7%,respectively, P<0.05）, with the highest in group C and the lowest in group A. The pos?
itive rate was significantly different in group A, B and C in TB BALF training (18.7%,56.6% and 87.8%, respec?
tively, P<0.05) and among them the difference was significant, with the highest in group C and the lowest in
group A. The same result was found in TB spurum training(positive rates of group A, B and C were 26.7%,
65.1% and 82.9%, respectively). Conclusion: The positive rates of BALF smearing, BALF training, and sputum
training increased with the rise of TB-DNA copy number in BALF. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
