| 斯伟宏,卢根林,黄惊鸿,吴爱兵.血硫化氢与肠缺血-再灌注损伤大鼠凝血功能的相关性研究 斯伟宏 卢根林 黄惊鸿 吴爱兵[J].浙江中西医结合杂志,2014,24(1): |
| 血硫化氢与肠缺血-再灌注损伤大鼠凝血功能的相关性研究 斯伟宏 卢根林 黄惊鸿 吴爱兵 |
| Correlation between hydrogen sulfide and coagulation function in Wistar rat with gut ischemia-reperfusion injury * SI Weihong, LU Genlin,,HUANG Jinghong,WU Aibing*The First Department of General Surgery, Yiwu Central Hospital, Yiwu 322000, China. |
| 投稿时间：2013-07-07 修订日期：2013-12-01 |
| DOI： |
| 中文关键词: 缺血-再灌注损伤 硫化氢 凝血功能 |
| 英文关键词:Ischemia-reperfusion injury Hydrogen sulfide Coagulation function |
| 基金项目:国家自然科学基金青年科学(81201672),义乌市引进人才立项项目(2012 -R-04) |
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| 中文摘要: |
| 【】 目的 ： 观察硫化氢与肠缺血-再灌注损伤大鼠凝血功能的相关性。方法： 雄性Wistar大鼠24只，分为S(假手术)组、I（缺血-再灌注）组,N（缺血-再灌注 NaHS）组，每组8只。S组仅行剖腹及游离肠系膜上动脉（SMA），I 和N组剖腹、游离及钳夹肠SMA 60min和再灌注120min，建立大鼠肠缺血-再灌注损伤模型。N组于再灌注前10min静脉注射NaHS 100μmol/kg后，按1mg(kg/ h)持续静脉注射直到再灌注2h，S和I组静脉注射相同体积的生理盐水，作用时间和持续时间均同N组。留取血测定H2S、凝血功能指标。结果: N组凝血酶原时间（prothrombin time，PT）、活化部分凝血酶时间（activated partial thromboplastin time，APTT)、凝血酶时间（thrombin time ，TT）、D-二聚体均低于I组、高于S组（p＜0.01），N组H2S、纤维蛋白原（fibrinogen，FIB）FIB低于S组、高于I组（p＜0.01）。H2S与FIB呈正相关（r= 0.758, p＜0.01），与PT 、APTT、TT、D-二聚体呈负相关（r= -0.715、-0.721、-0.689、-0.748, p＜0.01）。结论 H2S能有效纠正肠缺血-再灌注损伤大鼠凝血功能障碍。 |
| 英文摘要: |
| 【】Objective To study correlation between hydrogen sulfide and coagulation function in Wistar rat with gut ischemia-reperfusion injury. Methods Wistar rats were randomly divided into three groups(8 in each group): S(sham),I (ischemia-reperfusion), N (ischemia- reperfusion and sodium hydrosulfide).Rats in Group N were received sodium hydrosulfide (100μmol/kg bolus 1mg.kg-1.h-1 infusion) 10min prior to the onset of reperfusion, whereas rats in group S and I were received normal sodium of equal volume. Blood was used to detect hydrogen sulfide and haemorheology parameter. Results H2S,FIB in group N were significantly higher than those in group I, predominantly lower than those in group S（p＜0.01）. PT,APTT,TT,D-dimer in group N were predominantly higher than those in group S,strikingly lower than those in group I（p＜0.01）. H2S negatively correlates with PT,APTT,TT,D-dimer（r= -0.715,-0.721,-0.689,-0.748, p＜0.01）, postively correlating with FIB（r= 0.758, p＜0.01）.Conclusion H2S plays an important role in improving coagulation disorders in Wistar rat with gut ischemia-reperfusion injury. |
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