| 王少娟,林筱洁,尹利明,高瑞兰.七叶皂苷钠诱导人单核白血病SHI-1细胞凋亡的研究[J].浙江中西医结合杂志,2014,24(1): |
| 七叶皂苷钠诱导人单核白血病SHI-1细胞凋亡的研究 |
| Study on the Apoptosis-inducing Effects of Sodium Aescinate in Human Monocytic Leukemic SHI-1 Cells WANG Shaojuan,LIN Xiaojie,YIN Liming,etal. Zhejiang University of Traditional Chinese Medicine, Hangzhou(310053), China |
| 投稿时间：2013-07-21 修订日期：2013-10-14 |
| DOI： |
| 中文关键词: 七叶皂苷钠 SHI-1细胞 凋亡 |
| 英文关键词:Sodium aescinate SHI-1 cell apoptosis |
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| 中文摘要: |
| 目的：研究七叶皂苷钠对人急性单核白血病细胞株SHI-1细胞的抑制增殖及诱导凋亡的研究。方法：SHI-1细胞经不同浓度的七叶皂苷钠处理后,MTT法分析七叶皂苷钠对SHI-1细胞增殖的抑制作用,AnnexinⅤ/PI染色流式细胞术(FCM)检测细胞凋亡率,DNA凝胶电泳观察凋亡特异性DNA降解梯形条带,半定量PCR检测凋亡特异性酶Caspase-3 mRNA的表达水平。结果：MTT法显示七叶皂苷钠能够显著地抑制SHI-1细胞的增殖,呈时间和剂量依赖性(P<0.05)。经不同浓度的七叶皂苷钠处理24h,AnnexinⅤ/PI染色显示AnnexinⅤ阳性的凋亡细胞率明显上升,DNA琼脂糖凝胶电泳分析出现凋亡特异性的DNA降解梯形条带,半定量PCR显示Caspase-3 mRNA的表达水平随药物浓度的增加而升高(P<0.05)。 结论：七叶皂苷钠对SHI-1细胞具有抑制增殖和诱导凋亡的作用,本文为其抗白血病作用提供了实验依据。 |
| 英文摘要: |
| Objective To investigate the effects of sodium aescinate on both proliferation- inhibiting and apoptosis- inducing in human acute monocytic leukemic SHI-1 cells. Methods The effects of inhibition proliferation was detected by MTT method in sodium aescinate treated SHI-1 cells, the apoptosis rates of SHI-1 cells were analyzed by flow cytometry after AnnexinⅤ/PI staining. The gel electrophoresis of DNA ladder was used to analyze the bands of DNA degradation, and the expression level of Caspase-3 mRNA was tested by reverse transcription-polymerase chain reaction (RT-PCR). Results MTT assay showed that the proliferation of SHI-1 cells was effectively inhibited by sodium aescinate, in a time-and-does dependent manner(P<0.05). The AnnexinⅤ positive rate of SHI-1 cells after treated by sodium aescinate was significantly increased by FCM analysis(P<0.05). The apoptosis typical DNA fragments of SHI-1 cells induced by sodium aescinate was presented on the gel electrophoresis. The expression level of Caspase-3 mRNA was up regulated with increasing concentration of sodium aescinate by RT-PCR detection(P<0.05). Conclusion Sodium aescinate is an efficacy Chinese medicine extraction from herb of Aesculus wilsonii Rehd to suppress proliferation and induce apoptosis in SHI-1 leukemia cells, our results provides an experimental evidence for the activity of sodium aescinate on anti-leukemia. |
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