| 黄美春,胡刚,朱芸芸,丁慧登,鲁盈.三七总皂苷对HB-EGF诱导的系膜细胞增殖作用研究[J].浙江中西医结合杂志,2013,23(11):886-888 |
| 三七总皂苷对HB-EGF诱导的系膜细胞增殖作用研究 |
| Effect of Panax Notoginseng Saponins on proliferation of mesangial cell induced by HB-EGF |
| 投稿时间：2013-06-05 |
| DOI： |
| 中文关键词: HB-EGF 系膜细胞 细胞增殖 三七总皂苷 |
| 英文关键词:HB-EGF mesangial cells cell proliferation panax notoginseng saponins |
| 基金项目: |
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| 摘要点击次数: 1260 |
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| 中文摘要: |
| 目的：观察三七总皂苷对肝素结合性表皮生长因子样生长因子（HB-EGF）诱导大鼠肾小
球系膜细胞增殖的作用。方法：以原代培养大鼠肾小球系膜细胞为细胞模型，以400ng/mL 三七总
皂苷和100ng/mL HB-EGF为干预因素，实验分对照组、100ng/mL HB-EGF组和400ng/mL三七总
皂苷+100ng/mL HB-EGF组，刺激系膜细胞24、36、48h，采用MTT方法，观察三七总皂苷对重组
HB-EGF诱导系膜细胞增殖的作用情况。结果：HB-EGF刺激36h 时，系膜细胞明显增殖，与对照组
比较，P<0.05；三七总皂苷能抑制HB-EGF诱导的系膜细胞的增殖，与HB-EGF组比较，P<0.05。随
着刺激时间的延长，三七总皂苷对HB-EGF 诱导系膜细胞增殖的抑制作用增强，但36h 与24h 比
较，差异无统计学意义（P>0.05）；与48h 比较，差异有统计学意义（P<0.05）。结论：三七总皂苷能抑
制HB-EGF诱导的系膜细胞增殖作用，且作用随着刺激时间延长而增强。三七总皂苷可能通过抑
制HB-EGF对系膜细胞增殖作用而延缓肾小球硬化。 |
| 英文摘要: |
| Objective：To investigate the influence of panax notoginseng saponins（PNS）on proliferation of mesangial
cell induced by heparin-binding epidermal growth factor like growth factor（HB-EGF）and its mechanism in kidney
disease. Methods：The primary cultured SD rat mesangial cell were used as cell models. The cells were divided
into control group，100 ng/ml HB-EGF group，and 400 ng/ml PNS+100 ng/ml HB-EGF group，and were cultured
with these solutions for 24，36，and 48 h，respectively. Cell proliferation was assessed by MTT colorimetric assay.
Results：HB-EGF stimulated the proliferation of mesangial cells in 36 h（compared with control group P<0.05）but
the effect was inhibited by PNS（compared with HB-EGG group P<0.05），and the effect of inhibition in 48 h was
more obvious than that in 36 h（P<0.05）. But no significant difference in the effect of inhibition was noted between
cells treated for 36 h and 24 h（P>0.05). Conclusion：PNS can inhibit the proliferation of mesangial cells induced
by HB-EGF，and the effect of inhibition gradually increases with the prolongation of the culture time. PNS may re-
tard the development of glomerular sclerosis by inhibiting the proliferation of mesangial cells induced by HB-EGF. |
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