| 刘颖慧,周旦阳,寿成珉,牟新.杭白菊黄酮对高糖诱导的人脐静脉内皮细胞的影响* [J].浙江中西医结合杂志,2014,24(7): |
| 杭白菊黄酮对高糖诱导的人脐静脉内皮细胞的影响* |
| The influence of flavonoids from Chrysanthemum morifolium on the apoptosis of HUVEC |
| 投稿时间：2013-11-20 修订日期：2014-04-18 |
| DOI： |
| 中文关键词: 杭白菊 糖尿病 细胞 培养 凋亡 |
| 英文关键词:Chrysanthemum diabetes morifolium cell culture apoptosis |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨杭白菊黄酮对高糖诱导的人脐静脉内皮细胞(HUVEC)凋亡的影响,分析杭白菊黄酮对血管内皮细胞的保护作用,为糖尿病并发症治疗的新药开发提供理论基础。方法：分正常对照组、高糖浓度组、不同浓度黄酮组,实验后作用24~72h,用显微镜观察细胞形态改变并通过MTT法检测细胞活力。结果：MTT法检测结果表明不同浓度杭白菊黄酮组细胞存活率均显著高于高糖组(p<0.05),尤以400mg.mL-1的药物作用最为显著。结论：杭白菊黄酮对高糖诱导的HUVEC细胞凋亡有明显的抑制作用,且存在剂量依赖性,对血管内皮具有保护作用。 |
| 英文摘要: |
| Objective: The purpose is to discuss the influence of flavonoids from Chrysanthemum morifolium on the apoptosis of HUVEC, discuss the flavonoids from Chrysanthemum morifolium on protecting vascular endothelial cells. Method: Dividing normal control group, high glucose groupd and flavone of different concentrations group, after dealing with 24-72h, observed the morphological changes of cells by microscope and cell proliferation was measured by MTT chromatometry. Result: Different concentrations of flavonoids from Chrysanthemum morifolium significantly improved the cell viability and decreased the apoptosis rate of high glucose-induced HUVEC, with a maxminal effect at 400mg.mL-1. Conclusion: For the apoptosis of high glucose-induced cells, the flavonoids from Chrysanthemum morifolium has different degree of inhibition, however, it has protective effects on vascular endothelial cells. |
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