| 詹欣,张玲云,王志华.茶多酚对卵巢癌SKOV3细胞自噬水平的影响及相关机制研究[J].浙江中西医结合杂志,2015,25(1): |
| 茶多酚对卵巢癌SKOV3细胞自噬水平的影响及相关机制研究 |
| The effect of EGCG-induced autophagy of person ovarian cancer cell line SKOV3 and underline mechanism |
| 投稿时间：2014-04-12 修订日期：2014-11-11 |
| DOI： |
| 中文关键词: 自噬 人卵巢癌SKOV3细胞 茶多酚 AKT |
| 英文关键词:autophagy the person ovarian cancer cell line SKOV3 (?)-epigallocatechin-3-gallate (EGCG) AKT |
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| 中文摘要: |
| 目的：研究茶多酚(EGCG)对人卵巢癌SKOV3细胞自噬水平的影响及相关机制。方法：用EGCG处理SKOV3细胞,Western blot检测自噬相关蛋白LC3-II及AKT信号通路相应蛋白表达的变化。结果：EGCG处理SKOV3细胞后,自噬相关蛋白LC3-II的表达上调,并呈一定的时间浓度依赖性。EGCG处理SKOV3细胞后,蛋白激酶B(AKT)的磷酸化水平下调,AKT激活剂胰岛素样生长因子1预处理后,自噬相关蛋白LC3-II的增高表达被抑制。结论：EGCG通过AKT介导的信号通路诱导SKOV3细胞自噬水平升高。 |
| 英文摘要: |
| AIM: To investigate the effect of EGCG-induced autophagy of person ovarian cancer cell line SKOV3 and explore the mechanism. METHODS: After cultured person ovarian cancer cell SKOV3 were treated with EGCG, LC3-II and AKT protein expression was detected by Western blot; RESULTS: After cultured person ovarian cancer cell SKOV3 were treated with EGCG, the level of autophagy-associated protein LC3-II was up-regulated; The level of phospho-AKT was also decreased by treatment with EGCG. Pretreatment with AKT activator, insulin-like growth factor 1 (IGF-1), suppressed the EGCG-induced LC3-II expression. CONCLUSION: EGCG can induce autophagy in person ovarian cancer cell SKOV3 through AKT signaling pathway. |
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