| 石峻,周冰之,宋珈,王俊.灯盏花素对糖基化终产物条件下心肌细胞缝隙连接蛋白下降的影响及相关机制研究[J].浙江中西医结合杂志,2014,24(12): |
| 灯盏花素对糖基化终产物条件下心肌细胞缝隙连接蛋白下降的影响及相关机制研究 |
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| 投稿时间：2014-04-25 修订日期：2014-09-03 |
| DOI： |
| 中文关键词: 灯盏花素 糖基化终产物 connexin 43 心肌细胞 |
| 英文关键词:breviscapine advanced glycation end products connexin 43 cardiomyocytes. |
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| 中文摘要: |
| 目的：研究灯盏花素对糖基化终产物（AGEs）条件下心肌细胞缝隙连接蛋白Cx43的影响及相关机制。方法：出生3天SD乳鼠心肌细胞原代培养，用AGEs处理心肌细胞，相同条件下牛血清白蛋白（BSA）处理为对照组，Western blot检测不同条件下Cx43，细胞外调节蛋白激酶（ERK），磷酸化细胞外信号调节激酶（p-ERK）蛋白表达变化。结果：AGEs处理心肌细胞后，缝隙连接蛋白Cx43表达量下降，丝裂原活化蛋白激酶（MAPK）信号通路中ERK磷酸化水平升高，而灯盏花素能够抑制AGEs诱导的缝隙连接蛋白Cx43表达量下降，同时能够抑制ERK磷酸化水平。结论：灯盏花素对AGEs诱导缝隙连接蛋白Cx43表达量下降有保护作用，这可能与灯盏花素抑制ERK信号通路激活相关。 |
| 英文摘要: |
| AIM: To investigate the effect and mechanisms of breviscapine on reduced Cx43 expression by advanced glycation end products in rat cardiomyocytes. METHODS: Cardiomyocytes were obtained from 3 day old Sprague-Dawley rats. After cultured cardiomyocytes were treated with advanced glycation end products (AGEs) or BSA, Cx43, ERK and p-ERK expression was measured by Western blot. RESULTS: After cultured cardiomyocytes were treated with AGEs, the level of protein Cx43 was down-regulated. However, the protein Cx43 express was increased co-incubation with breviscapine in AGEs-treated group. The level of phospho-ERK were increased by treatment with AGEs. Pretreatment with breviscapine can suppressed AGE-induced ERK phosphorylation. |
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