| 侯兆阳,陈哲,魏家森.补阳还五汤在急性脊髓损伤中的治疗作用[J].浙江中西医结合杂志,2015,25(1): |
| 补阳还五汤在急性脊髓损伤中的治疗作用 |
| Therapeutic effects of Buyang Huanwu Decoction after acute spinal cord injury |
| 投稿时间：2014-04-29 修订日期：2014-10-29 |
| DOI： |
| 中文关键词: 脊髓损伤 补阳还五汤 细胞凋亡 VEGF |
| 英文关键词:ASCI BYHWD Apoptosis VEGF |
| 基金项目:浙江省中医药科技计划项目、浙江省教育厅科研项目、浙江省医药卫生科技计划项目 |
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| 中文摘要: |
| 目的 观察补阳还五汤（BYHWD）在急性脊髓损伤（ASCI）中的治疗作用及潜在机制。方法 SD 大鼠随机分为空白组、损伤对照组、BYHWD治疗组，采用ALLEN’S打击法建立急性脊髓损伤模型，各组分别于术后1、7、14d观察其行为学BBB评分，14d通过免疫组化的方法观察脊髓损伤部位神经细胞凋亡(caspase-3p20、Bcl-2与Bax)、血管内皮生长因子（VEGF）的表达水平。结果 （1）行为学观察评分：BYHWD治疗组与损伤对照组下肢功能均有恢复(P＜0.05），且BYHWD治疗组较损伤对照组恢复更明显(P＜0.05）；（2）免疫组化检测：caspsase-3阳性细胞数BYHWD治疗组表达较损伤对照组少(P＜0.05）；Bcl-2阳性细胞BYHWD治疗组的表达较损伤对照组多(P＜0.05）；Bax阳性的细胞BYHWD治疗组的表达较损伤对照组少(P＜0.05）；VEGF阳性的细胞BYHWD治疗组的表达较损伤对照组多(P＜0.05）。结论 BYHWD可提高脊髓损伤部位VEGF的表达，使细胞凋亡明显减少，从而促进功能的恢复的作用，因此BYHWD有可能成为治疗脊髓损伤的药物之一。 |
| 英文摘要: |
| Objective The aim of this study was to determine the therapeutic efficacy of Buyang Huanwu Decoction (BYHWD) treatment after acute spinal cord injury(ASCI) in rat and to investigate the underlying mechanism.Methods ASCI was induced using the modified weight-drop method in Sprague–Dawley rats. The SCI animals were randomly divided into three groups: sham group (laminectomy only) , vehicletreated group ,and additionally with BYHWD- treated group. Locomotors functional recovery was assessed during the 1、7、14 days post operation period by performing open-field locomotors tests.At the end of the study, the segments of spinal cord encompassing the injury site were removed for histopathological
analysis.Immunohistochemistry was performed to observe the expression of B-cell CLL/lymphoma-2 (Bcl-2),BCL-2-associated X protein(Bax) 、caspase-3 and vascular endothelial growth factor (VEGF).Results The BYHWD-treated animals had significantly better locomotor function recovery than the vehicletreated group(P＜0.05). In addition , Compared with the vehicletreated group,the BYHWD-treated had increased expression of Bcl-2(P＜0.05)and VEGF(P＜0.05), reduced expression of caspase-3(P＜0.05) and Bax (P＜0.05) after spinal cord injury.Conclusion These findings suggest that BYHWD treatment after SCI can significantly improve locomotor recovery, and this neuroprotective effect may be related to the upregulation of VEGF and the inhibition of neural apoptosis . Therefore , BYHWD may be useful as a promising therapeutic agent for ASCI. |
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