| 陈碧瑶,李斌,韦思明,王力.缺血后处理对大鼠睾丸缺血再灌注损伤的影响[J].浙江中西医结合杂志,2015,25(2): |
| 缺血后处理对大鼠睾丸缺血再灌注损伤的影响 |
| Effect of Ischemic Post-Conditioning on Testicular Ischemia-Reperfusion Injury in Rats CHEN Biyao, LI Bin, WEI Siming, WANG Li. Department of Clinical Medicine, Zhejiang Medical College, Hangzhou (310053), China |
| 投稿时间：2014-09-07 修订日期：2014-09-07 |
| DOI： |
| 中文关键词: 缺血后处理 睾丸缺血再灌注损伤 睾丸扭转复位 |
| 英文关键词:ischemic post-conditioning testicular ischemia-reperfusion injury testicular torsion-detorsion |
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| 中文摘要: |
| 目的 睾丸扭转复位后的损伤是一个典型的缺血再灌注损伤。本研究探讨了缺血后处理对大鼠睾丸缺血再灌注损伤的影响。方法 60只成年雄性SD大鼠随机分为3组,每组20只。对照组承受了左侧睾丸假手术。在缺血再灌注组,左侧睾丸扭转720°,2小时后复位。在缺血后处理组,左侧睾丸扭转720°,2小时后行缺血后处理,即先将睾丸复位,血流再灌注10秒,然后将睾丸再次扭转720°,使睾丸缺血10秒,反复6个循环。最后睾丸复位,血流持续灌注。复位后4小时,每组一半的大鼠被处死,行左侧睾丸切除术,测定丙二醛水平(活性氧标志)。复位后3个月,处死剩余的大鼠,行左侧睾丸切除术,分析睾丸生精功能。结果 睾丸扭转复位引起睾丸内丙二醛水平明显升高,睾丸生精功能显著下降。缺血后处理明显降低了睾丸内丙二醛水平,显著提高了睾丸生精功能。结论 缺血后处理对睾丸缺血再灌注损伤有保护作用,该作用与减少活性氧产生有关。 |
| 英文摘要: |
| Objective The damage after testicular torsion-detorsion is a typical ischemia-reperfusion injury. This study investigated effect of ischemic post-conditioning on testicular ischemia-reperfusion injury in rats. Methods A total of 60 adult male Sprague-Dawley rats were randomly divided into three groups, each containing 20 rats. The control group underwent a sham operation of the left testis. In the ischemia-reperfusion group, the left testis was rotated 720° for 2 hours, and then detorsion was performed. In the ischemic post-conditioning group, the left testis was rotated 720° for 2 hours, and then ischemic post-conditioning was performed. First, testicular detorsion was done for 10-second blood flow reperfusion. Then, left testis was rotated 720° again for 10-second ischemia. Such procedures were repeated 6 times. Lastly, testicular detorsion was performed for persistent blood flow reperfusion. One half of the rats in each group were killed 4 hours after detorsion, and left orchiectomy was done for measurement of malondialdehyde level（mark of reactive oxygen species）. The remainder were killed 3 months after detorsion, and left orchiectomy was performed for analysis of testicular spermatogenesis. Results Testicular torsion-detorsion caused a significant increase in the malondialdehyde level in the testes, and a significant decrease in testicular spermatogenesis. Ischemic post-conditioning significantly decreased malondialdehyde level in testes, and improved testicular spermatogenesis. Conclusion Ischemic post-conditioning has a protective effect on testicular ischemia-reperfusion injury. The effect is related to reduction in reactive oxygen species production in testes. |
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