| 韦丽玲,朱美飞,黄立权,江荣林,王灵聪.姜黄素对人支气管上皮细胞MMP-9的影响[J].浙江中西医结合杂志,2015,25(9): |
| 姜黄素对人支气管上皮细胞MMP-9的影响 |
| Effects of curcumin on the expression of MMP-9 in human bronchial epithelial cells |
| 投稿时间：2014-11-24 修订日期：2015-06-18 |
| DOI： |
| 中文关键词: 姜黄素 人支气管上皮细胞 MMP-9 |
| 英文关键词:curcumin human bronchial epithelial cells MMP-9 |
| 基金项目:浙江省自然科学基金(Y207052,LY12H29005)； |
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| 中文摘要: |
| 【】目的：观察姜黄素(curcumin, cur)对人支气管上皮细胞(human bronchial epithelial cells, HBECs)MMP-9表达的影响。方法：体外培养人支气管上皮细胞,分为空白组、LPS组及姜黄素组,其中姜黄素组在相同浓度LPS刺激下又各分为5、10、20、40和80μmol／L 5个浓度组,作用4h后在显微镜下观察cur干预后细胞的形态变化,采用实时荧光定量PCR观察人支气管上皮细胞MMP-9 mRNA的表达变化。结果：显微镜下观察姜黄素干预后的细胞形态良好,细胞无明显损伤。实时PCR结果显示,空白组可见一定量MMP-9 mRNA表达,与空白组比较,LPS组的人支气管上皮细胞MMP-9 mRNA表达量明显增加,80μmol／L cur亦可增加MMP-9 mRNA的表达(p<0.05)。与LPS组比较,10μmol／L cur组人支气管上皮细胞的MMP-9 mRNA表达量显著下降(p<0.05),5μmol／L、20μmol／L、40μmol／L的cur对支气管上皮细胞MMP-9的mRNA表达无明显作用。结论：10μmol／L姜黄素可降低人支气管上皮细胞的MMP-9mRNA的表达。 |
| 英文摘要: |
| Objective:To observe the role of curcumin (cur) on the expression of MMP-9 in human bronchial epithelial cells(HBECs). Methods:HBECs was cultured in vitro and divided into blank group,LPS group and cur groups,and cur groups was set at 5 concentrations of 5,10,20,40,80μmol / L after the stimulation of the same LPS concentration .Observe the morphological changes of cells through a microscope after cur intervention and real-time quantitative PCR was performed to examine the dynamic expression of MMP-9 in human bronchial epithelial cells after 4 hours.Results:The cells’ morphology were well without significant damage after cur intervention by means of the microscope.The consequences of real-time PCR show that the blank group could produce a certain amount of MMP-9 expression,and compared with it,the group of LPS has a significantly increasing in the expression of MMP-9 in the human bronchial epithelial cells as well as the subgroup of 80μmol／L cur (p <0.05).Compared with the group of LPS,the concentration subgroup of 10μmol / L cur’ expression of MMP-9 in human bronchial epithelial cells decreased significantly(p <0.05), the concentration of 5μmol / L, 20μmol / L, 40μmol / L of cur has no marked effect.Conclusion:10μmol／L of Curcumin can lower the expression of MMP-9 in the human bronchial epithelial cells. |
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