| 金良.人类卵子改良程序化冷冻和玻璃化冷冻的比较研究[J].浙江中西医结合杂志,2015,25(9): |
| 人类卵子改良程序化冷冻和玻璃化冷冻的比较研究 |
| Comparison of human oocytes cryopreservation by modified slow-freezing and vitrification |
| 投稿时间：2015-01-20 修订日期：2015-03-02 |
| DOI： |
| 中文关键词: 程序化冷冻 玻璃化冷冻 复苏率 优质胚胎率 人类卵子 |
| 英文关键词:Slow-freezing, vitrification survival rate percentage of high-quality embryos human oocyte |
| 基金项目: |
|
| 摘要点击次数: 1350 |
| 全文下载次数: 0 |
| 中文摘要: |
| 【】目的：研究两种冷冻方法对人类卵子冻融结果的影响。方法：分为程序化冷冻卵子与玻璃化冷冻卵子两组,对于卵子冻融后的复苏率、受精率以及优质胚胎率等指标进行比较。结果：程序化冷冻组卵子的复苏率(71.05%,108/152)显著低于(P<0.05)玻璃化冷冻组(81.75%,103/126),但优质胚胎率(41.07%,23/56)显著高于(P<0.05)玻璃化冷冻组(30.23%,13/53),而受精率、卵裂率及囊胚形成率无统计学差异。结论：玻璃化冷冻卵子可以获得较高的复苏率,但使用改良程序化冷冻试剂盒冻融卵子,可能较玻璃化冷冻获得更好发育潜能。 |
| 英文摘要: |
| 【】Objective: To compare the effect of cryopreservation of human oocytes with slow-freezing and vitrification. Method：To compare the survival, fertilization, early embryonic development in frozen-thawed human oocytes after slow-freezing and vitrification.Results:the oocyte survival rate was statistically significantly lower in the slow-freezing group (108 out of 152,71.05%) than the vitrification group(103 out of 126,81.75%),but there was a considerable difference in the percentage of high-quality embryos between slow-freezing and vitrification groups: 23 out of 56 (41.07%) versus 13 out of 53(30.23%), respectively(P<0.05). The fertilization rate and the percentage of cleavage and blastocyst development were not statistically significantly.Conclusion：Vitrification is superior to the slow-freezing method, leading to improved oocyte survival rate,but using modified slow-freezing for oocyte cryopreservation may get better embryonic development potential. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
