| 陈琳瑶,阮丹,王跃鑫,黄巧玲.黄芪多糖对 Caco-2细胞上P-gp功能、表达的影响[J].浙江中西医结合杂志,2015,25(9): |
| 黄芪多糖对 Caco-2细胞上P-gp功能、表达的影响 |
| Effect of astragalus polysaccharide on the function and expression of p-glycoprotein in Caco-2 cells |
| 投稿时间：2015-03-16 修订日期：2015-05-12 |
| DOI： |
| 中文关键词: 黄芪多糖 P糖蛋白 Caco-2细胞 |
| 英文关键词:APS p-glycoprotein Caco-2 cell |
| 基金项目:浙江省中西医结合学会科研项目(2013LYZD016) |
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| 中文摘要: |
| 【目的】 研究黄芪多糖(astragalus polysaccharide,APS)对Caco-2细胞上P糖蛋白(P-gp)功能、表达的影响。 【方法】 采用MTT法考察药物对细胞的毒性,确定药物最大无毒浓度；流式细胞仪测定Caco-2细胞上P-gp底物罗丹明-123(Rh-123)和抗体的荧光强度,评价APS对P-gp功能和表达的影响；建立Caco-2细胞单层模型,研究APS对Rh-123跨膜转运的影响。【结果】 细胞毒性实验结果显示,APS在0.1～100 μg.mL-1浓度范围内对Caco-2细胞无明显毒性,细胞存活率＞90%；不同浓度的APS作用后,增加了细胞内Rh-123的蓄积,对P-gp的功能表现为抑制作用；中、高浓度组APS对P-gp的表达有抑制作用,其表达量分别降低了11.23%和16.41%；APS高浓度组明显抑制了Rh-123的双向跨膜转运,对P-gp有抑制作用。【结论】 黄芪多糖(APS)对P-gp的功能和表达有一定的抑制作用,且在高浓度尤为明显,能显著增加Rh-123在Caco-2细胞内的蓄积,并抑制其双向跨膜转运。 |
| 英文摘要: |
| Objective To explore the effect of astragalus polysaccharide(APS) on the function and expression of p-glycoprotein in Caco-2 cells. Methods The cytotoxicity of different drug concentrations was measured by MTT assay, in order to confirm the maximum non-toxic concentration of drug. The flow cytometry was used to detect the fluorescence of rhodamine 123 concentration and antibody concentration. The Caco-2 cell monolayer was built to evaluate the effect of APS on the bi-directional transports of Rh-123. Results The cell viability of Caco-2 cells was higher than 90% when the concentration of astragalus polysaccharide was between 0.1 and 100μg.mL-1with MTT. Compared with the control groups, APS increased the cellular Rh-123 concentration. The expression levels of p-gp in Caco-2 cells were by 11.23% and 16.41%, when treated with APS at intermediate and high concentration groups, respectively. Rh-123 bi-directional transport experiment showed that the p-gp level was obviously inhibited after 72 h incubation at the high concentration group. Conclusion APS is an inhibitor of P-gp which can inhibit the expression and function of P-gp in Caco-2 cells, also can reduce the bi-directional transport of Rh-123. More importantly, the inhibitive effect of APS is concentration-dependent. |
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