| 张弦.吴茱萸碱诱导人胃癌SGC7901细胞凋亡及机制研究[J].浙江中西医结合杂志,2016,26(3): |
| 吴茱萸碱诱导人胃癌SGC7901细胞凋亡及机制研究 |
| Apoptosis of SGC7901 gastric cancer cell induced by evodiamine and the primary mechanisms |
| 投稿时间：2015-08-10 修订日期：2015-10-19 |
| DOI： |
| 中文关键词: 吴茱萸碱 胃癌 凋亡 Survivin Caspase-3 |
| 英文关键词:evodiamine gastric cancer apoptosis Survivin Caspase-3 |
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| 中文摘要: |
| 目的 观察吴茱萸碱对胃癌SGC7901细胞的抑制作用,探讨可能的分子机制。方法 体外培养人胃癌SGC7901细胞,用不同浓度的吴茱萸碱作用于SGC7901细胞24h。MTT比色法观察吴茱萸碱对SGC7901细胞增殖活性的影响；倒置相差显微镜及荧光电镜观察细胞凋亡状态；流式细胞仪检测SGC7901细胞凋亡情况；用RT-PCR检测不同浓度的吴茱萸碱干预24h后SGC7901细胞Survivin和Caspase-3的mRNA表达。结果 吴茱萸碱能抑制SGC7901细胞增殖,呈剂量依赖性；倒置相差显微镜及荧光电镜下均观察到典型的细胞凋亡状态；流式细胞仪显示吴茱萸碱可诱导SGC7901细胞凋亡,且随剂量增加作用增强；RT-PCR显示随吴茱萸碱浓度的增加,细胞内Survivin基因表达强度逐渐降低,Caspase-3表达强度逐渐增强。结论 吴茱萸碱能抑制人胃癌SGC7901细胞增殖并诱导其凋亡, SGC7901细胞中Survivin mRNA表达水平显著降低,从而可能下调其对Caspase-3的抑制作用,使细胞凋亡增加。 |
| 英文摘要: |
| Objective The apoptosis of SGC7901 human gastric cancer cells induced by evodiamine was studied in order to assess its antitumor effect and identify the molecular mechanism involved. Methods SGC7901 gastric cancer cells were cultured in vitro and treated by evodiamine of 0-48umol/L concentrations for 24 h. Inhibition on proliferation of SGC7901 cells was assessed by MTT assay. The morphology of treated SGC7901 cells was observed by optical microscope. Effect of evodiamine on the nuclear morphology of cells was analyzed using Hoechst 33258 staining by fluorescence microscope. Annexin V-FITC/PI staining and flow cytometric measurement were used for investigating the effect of evodiamine on induction of apoptosis in SGC7901 cells.Gene transcription of Survivin and Caspase-3 was also examined by RT-PCR. Results The proliferation of SGC7901 cells was obviously inhibited by evodiamine and apoptosis of SGC7901 was induced by a dose-dependent manner. Characteristic apoptosis were confirmed by optical microscope, and Hoechst 33258 staining analysis indicated that evodiamine caused typical characteristics of apoptotic programmed cell death, such as cell shrinkage, apoptotic body formation. Flow cytometric analysis demonstrated that evodiamine caused a dose-dependent apoptosis of SGC7901 cells. The transcription of Survivin gene showed decreasing tendency , and the Caspase-3 gene showed increasing tendency when treated by 0-48umol/L evodiamine for 24 h. Conclusion The proliferation of SGC7901 cells may be inhibited by evodiamine and its apoptosis is also induced by evodiamine. evodiamine down-regulated Survivin mRNA levels and up-regulated the expression of caspase-3, and activated the activities of caspase-3 in cellular and induced apoptosis in SGC7901 cells. |
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