| 韩知忖,刘冰,周辉.人参皂苷Rg1对神经细胞衰老和衰老相关异染色质聚集的影响[J].浙江中西医结合杂志,2016,26(4): |
| 人参皂苷Rg1对神经细胞衰老和衰老相关异染色质聚集的影响 |
| Effects of ginsenoside Rg1 on senescent nerve cells and senescence-associated heterochromatic foci |
| 投稿时间：2015-09-15 修订日期：2016-01-15 |
| DOI： |
| 中文关键词: 人参皂苷Rg1 细胞衰老 衰老相关异染色质聚集 |
| 英文关键词:ginsenoside Rg1 cellular senescence senescence-associated heterochromatic foci |
| 基金项目:浙江省自然科学基金资助项目（从组蛋白去甲基化酶探讨人参皂苷Rg1对神经细胞衰老的保护作用），项目编号：Y2100169 |
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| 中文摘要: |
| 目的：探讨人参皂苷Rg1预处理对三丁基过氧化氢(t-BHP)诱导的神经细胞衰老及异染色质聚集的影响。方法：常规培养Neuro-2a细胞株,在造模药物刺激前,给予不同剂量的人参皂苷Rg1,同时设置模型对照组、正常对照组。进行细胞活力检测、衰老相关半乳糖苷酶染色、衰老相关异染色质聚集检测。结果：人参皂苷Rg1预处理可改善t-BHP诱导的细胞活力降低,且此种改善呈剂量依赖性；人参皂苷Rg1预处理各组,SA-β-gal染色细胞阳性数呈剂量依赖性降低；人参皂苷Rg1预处理可预防t-BHP诱导的SAHF形成,呈显著剂量依赖性。结论：人参皂苷Rg1预处理可剂量依赖性地预防神经细胞衰老,防止异染色质聚集,有必要进一步研究。 |
| 英文摘要: |
| Objective ：To investigated whether Ginsenoside Rg1 prevents oxidative-stress-induced senescence and cellular senescence, senescence-associated heterochromatic foci(SAHF) in Neuro-2a cells. Methods：Senescent Neuro-2a cells induced by tBHP (t-butylhydroperoxide) were treated in advance with Ginsenoside Rg1, senescence-associated β-galactosidase(SA-beta-gal) activity, cell viability and SAHF were examined. Results：In a dose-dependent manner, Ginsenoside Rg1 enhanced cell viability, decreased the number of SA-beta-gal positive cells, and decreased SAHF formation. Conclusion：Our findings suggested that, Ginsenoside Rg1 protects N2a cells from tBHP-induced senescence, and decrease of SAHF play a critical role in the inhibition of senescence induction. |
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