| 殷一红,王小云,阳少辉.疏肝降肺法对“激怒”伤大鼠模型海马区神经递质及子宫、卵巢ERβ的影响[J].浙江中西医结合杂志,2016,26(9): |
| 疏肝降肺法对“激怒”伤大鼠模型海马区神经递质及子宫、卵巢ERβ的影响 |
| Effect of soothing liver and dropping lung on hippocampus neurotransmitter and uterus, ovaries ER beta of “Anger”Injuried Female Rat Model |
| 投稿时间：2015-09-25 修订日期：2016-07-07 |
| DOI： |
| 中文关键词: 疏肝降肺 神经递质 ERβ 动物实验 |
| 英文关键词:Soothing liver and dropping lung Neurotransmitter Estrogen receptorβ Animal experiment |
| 基金项目:浙江省中医药科技项目（2015ZA189) |
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| 摘要点击次数: 1150 |
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| 中文摘要: |
| 目的 探讨疏肝降肺法对“激怒”伤模型雌性大鼠的神经递质活性及子宫-卵巢雌激素受体的影响。方法 将雌性SD大鼠随机分为正常对照组、“怒”模型组、疏肝降肺汤组，每组8只。采用夹尾间接激怒法建立“怒”大鼠动物模型。疏肝降肺汤组给予相应中药灌胃（每只大鼠含生药7.56g?kg?d），正常组及模型组给予等量的生理盐水灌胃，共计4周。观察各组额叶皮质、海马组织神经递质及子宫-卵巢雌激素受体β含量差异。高效液相-电化学法测定神经递质包括去甲肾上腺素（Norepinephrine，NE）、多巴胺（Dopamine，DA）、五羟色胺（5-hudroxytryptamine，5-HT）含量及免疫组化法测定子宫、卵巢雌激素受体β（Estrogen receptor，ERβ）含量。结果 与正常组比较，模型组NE、DA、5-HT含量均增加，差异有统计学意义[NE：（78.538.64）ng/g比（37.3811.08）ng/g，P＜0.01；DA：（41.7811.26）ng/g比（21.5610.72）ng/g，P＜0.01；5-HT：（26.489.67）ng/g比（10.595.05）ng/g，P＜0.01]；与模型组比较，疏肝降肺汤组NE、DA、5-HT含量均减少，差异有统计学意义[NE：（36.8010.75）ng/g比（78.538.64）ng/g，P＜0.05；DA：（23.046.17）ng/g比（41.7811.26）ng/g，P＜0.05；5-HT：（12.2012.07）ng/g比（26.489.67）ng/g，P＜0.01]；疏肝降肺汤组大鼠对ERβ积分光密度增加有一定改善作用，但组间比较差异无统计学意义（P＞0.05）。结论 实验研究表明疏肝降肺法对“激怒”伤引起的神经递质紊乱及卵巢功能损伤有一定的调节改善作用，可能是临床治疗“怒”情志所致疾病的作用机制。 |
| 英文摘要: |
| Objective To study the method of soothing liver and dropping lung on "anger" injury model of female rat neurotransmitter activity and the influence of the uterus, ovarian estrogen receptor.Methods SD rats were randomly divided into Constant control group, "anger" model group, soothing liver and dropping lung group(n=8,respectively), another 10 rats were fed as invading rats.Model rats are established by clipping tail.Each group were given the corresponding Chinese Medicine（7.56g?kg?d） or the same capacity of saline for 4 weeks.Neurotransmitter in frontal cortex, hippocampus and estrogen receptor beta in uterus, ovarian were respectively detected after four weeks. NE、DA、5-HT were detected by HPLC-electrochemical method.Eestrogen receptor beta was detected by Immunohistochemical? method. Results Compared with normal group, NE, DA and 5 - HT contents in model group had statistically significant difference(NE：78.538.64ng/g vs 37.3811.08ng/g，P＜0.01；DA：41.7811.26ng/g vs 21.5610.72ng/g，P＜0.01；5-HT：26.489.67ng/g vs 10.595.05ng/g，P＜0.01) ;Statistically significant difference were observed in contents of NE, DA and 5-HT between model group and traditional chinese medicine group (NE：36.8010.75ng/g vs 78.538.64ng/g，P＜0.05；DA：23.046.17ng/g vs 41.7811.26ng/g，P＜0.05；5-HT：12.2012.07ng/g vs 26.489.67ng/g，P＜0.01);there was no statistically significant difference in hypothalamus - ovarian ER βintegral optical density between groups.Conclusion The method of soothing liver and dropping lung had better effect on a variety of neurotransmitter activity and the ovarian function caused by "anger". This maybe the potential mechanism of dealing with diseases caused by "anger". |
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