| 朱程芬,邱晓晓,卢帅军,程泾,程蕾,朱长玲.补肾益冲抗衰汤对卵巢储备功能下降大鼠血清AMH和INHB的影响[J].浙江中西医结合杂志,2016,26(8): |
| 补肾益冲抗衰汤对卵巢储备功能下降大鼠血清AMH和INHB的影响 |
| Influence of Bushen Yichong Kangshuai Decoction onAMH and INHB of Rats with Diminished Ovarian Reverse |
| 投稿时间：2015-10-08 修订日期：2016-01-11 |
| DOI： |
| 中文关键词: 补肾益冲抗衰汤 卵巢储备功能下降 抗苗勒管激素 抑制素B |
| 英文关键词:BSYCKSD diminished ovarian reserve AMH INHB |
| 基金项目:浙江省中医药科技计划(2011ZB143),温州市医药卫生科学研究项目(2011B053),温州市鹿城区科技计划项目(Ｙ12003) |
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| 中文摘要: |
| 目的：观察补肾益冲抗衰汤对卵巢储备功能下降模型大鼠血清内分泌及抗苗勒管激素(AMH)、抑制素B(INHB)的影响。方法：选取70只8周龄雌性SD大鼠为研究对象,采用雷公藤多苷片制作卵巢储备功能下降大鼠模型。通过阴道涂片观察动情周期,光镜下观察卵巢组织形态学变化,酶联免疫法检测血雌二醇(E2)、卵泡刺激素(FSH)、黄体生成素(LH)、AMH、INHB水平。结果：本方能维持动情周期,促进卵泡发育成熟、改善内分泌水平,升高AMH、INHB水平。结论：采用雷公藤多苷片能够制作卵巢储备功能下降大鼠模型,与人类卵巢储备功能下降(DOR)临床表现基本一致；补肾益冲抗衰汤能够改善DOR大鼠性激素水平,升高AMH、INHB水平,并减少闭锁卵泡,从而改善卵巢储备功能。 |
| 英文摘要: |
| Objective: To observe the influence of Bushen Yichong Kangshuai Decoction(BSYCKSD) on endocrine and anti-Mullererian (AMH) and inhibin B (INHB) expressions of diminished ovarian reserve model rats. Methods: 8-week old 70 healthy female rat as the research object,Tripterygium wilfordii was used to set up diminished ovarian reserve model in rats.The estrous cycle was determined through the observation of vaginal smear. The morphological changes of ovarian tissue was observed by light microscopic,ELISA method was for measuring serum estradiol (E2),follicle stimulating hormone(FSH),luteinizing hormone(LH),AMH and INHB. Results: This prescription can maintain the estrous cycle,promote the development of mature follicles,improve endocrine levels and increase the level of AMH, of INHB. Conclusion: The diminished ovarian reserve model rats set up with Tripterygium wilfordii is corresponding to DOR typical performance of human;BSYCKSD can improve DOR sex hormone levels in rats,increase the level of AMH, of INHB,reduce the follicular atresia,and enhance the reserve function of ovary. |
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