| 陆一丹,徐良额,裘嘉琪,毛丹漪,刘晓谷,王大维.水飞蓟素逆转人乳腺癌耐药细胞株MCF-7/ADM耐药性的实验研究[J].浙江中西医结合杂志,2016,26(2): |
| 水飞蓟素逆转人乳腺癌耐药细胞株MCF-7/ADM耐药性的实验研究 |
| Reversal Effect of Silymarin on Multidrug Resistance Breast CancerCell MCF-7/ADM |
| 投稿时间：2015-10-20 修订日期：2015-11-21 |
| DOI： |
| 中文关键词: 乳腺癌 水飞蓟素 逆转耐药 CCK-8 |
| 英文关键词:Breast carcinoma Silymarin Reversing drug-resistance CCK-8 |
| 基金项目:浙江省大学生科技创新活动计划(新苗人才计划)项目（ 2014R410038）浙江省中医药科技计划项目（2016ZB030） |
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| 中文摘要: |
| 目的 明确水飞蓟素(Silymarin，Sily) 对人乳腺癌耐药细胞株MCF-7/ADM的逆转耐药作用。方法 以CCK-8法测定阿霉素（Adriamycin，Adm）对人乳腺癌敏感细胞株MCF-7/S和耐药细胞株MCF-7/ADM的毒性作用，计算出耐药倍数。以无细胞毒性的Sily（10μg/ml）作为逆转耐药剂，联合Adm观察其对耐药细胞株MCF-7/ADM的逆转耐药作用，计算得逆转倍数。结果 ①Adm对MCF-7/S和MCF-7/ADM的半数抑制浓度(IC50)分别为1.773 μg/ml和43.812 μg/ml，耐药倍数为24.7倍。②Sily能够增强ADM对MCF-7/ADM的细胞毒作用。以10μg/ml（抑制率为2.0%）的Sily联合Adm作用于MCF-7/ADM 48h后，耐药细胞株的IC50降至7.798 μg/ml，逆转倍数为5.6倍(P <0.01)。结论 Sily能够逆转人乳腺癌耐药细胞株MCF-7/ADM的耐药性。 |
| 英文摘要: |
| Objective To explore the effect of Silymarin on Adriamycin resistance of breast cancer cell line. Methods To measure toxicant effect of Adm to MCF- 7/S (sensitive strain) and MCF-7/ADM (multidrug- resistant strain) for resistant index by CCK-8 in vitro ; to observe the reversal effect of Silymarin （10μg/ml） which has screened for non-toxicant to MCF- 7/ADM by CCK-8. Results ① IC50 of Adm to MCF-7/S and MCF-7/ADM were 1.773 μg/ml and 43.812 μg/ml respectively. Drug-Resistant index of MCF-7/ADM was 24.7. ② Silymarin could increase cell toxic effect of Adm. After treated with 10μg/ml silymarin （Inhibition rate was 2.0%） combining with Adm to MCF- 7/ADM for 48 hours , cell’s IC50 declined to 7.798 μg/ml, and reversal index is 5.6 (P < 0.01 ). Conclusion Silymarin could reduce partly reverse multidrug- resistance to Adm of MCF- 7/ADM in vitro. |
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