| 王苗娟.抗肝癌合剂对人肝癌细胞凋亡及间隙连接通讯功能的影响[J].浙江中西医结合杂志,2016,26(9): |
| 抗肝癌合剂对人肝癌细胞凋亡及间隙连接通讯功能的影响 |
| The Effection of IPAH on Hepatocarcinomor cells of apoptosis and Gap junction intercellular communication (GJIC) |
| 投稿时间：2015-11-04 修订日期：2016-07-18 |
| DOI： |
| 中文关键词: 抗肝癌合剂 肝癌 细胞凋亡 细胞间通讯（GJIC) |
| 英文关键词:Instant Powder of Anti-hepatocarcinomoe (IPAH),Apoptosis,Gap junction intercellular communication(GJIC) |
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| 中文摘要: |
| 目的：探讨中药抗肝癌合剂(IPAH)含药血清对人肝癌细胞株 SMMC-7721 细胞凋亡和间隙连接通讯功能的影响。方法:制备抗肝癌合剂含药血清，分为高、中、低剂量组，另设阴性对照组和空白血清对照组。培养人肝癌细胞株 SMMC-7721 进行体外实验，流式细胞仪检测细胞凋亡情况，荧光漂白恢复实验（FRAP）观察对细胞间隙连接通讯功能的影响。结果：（1）抗肝癌合剂中、高剂量组可诱导人肝癌细胞系SMM C-7721细胞凋亡，与阴性对照组及空白对照组比较，差异有显著性（P＜0.01）。（2）抗肝癌合剂各剂量组均可明显增强人肝癌细胞系SMMC-7721的间隙连接通讯功能，荧光强度明显恢复，平均荧光恢复率明显增高，统计学分析差异有显著性，P均＜0.01。结论：抗肝癌合剂具有诱导肝癌细胞系SMMC-7721细胞凋亡的作用，呈一定的剂量和时间依赖关系。其作用可能与其上调细胞间隙连接通讯功能相关。 |
| 英文摘要: |
| Objective: Investigating the effect of IPAH on Hepatocarcinomor cells line SMMC-7721 of induced apoptosis and up-regulated the function of Gap junction intercellular communication (GJIC). Methods: Preparatinon drug-contained Serum of rabbits,In vitro experiments were carried out on hepatocarcinoma cells line SMMC-7721. The apoptosis was detected by flow cytometry, and the effect of fluorescence recovery experiment (FRAP) on cell gap junction communication was observed. Redults: Compared with controlled groups,middle dose group and higher dose group induced cell apoptosis singnificantly(P＜0.01). Compared with controlled groups, the intensify of the three different concentration drug-contained serum group increase conspicuously,the rate f the fluorescence recovery arose drastically.It showed to be different singnificantly(P＜0.01). Conclusion: IPAH can induce the hepatocarcinoma cells line SMMC-7721 apoptosis,and there are dose-depended and time-depended.Its function may be related to up-regulated the function of GJIC. |
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