| 章艳斐.桔梗皂苷D通过促进DISC的形成增强TRAIL对肺癌细胞的凋亡诱导效应[J].浙江中西医结合杂志,2016,26(8): |
| 桔梗皂苷D通过促进DISC的形成增强TRAIL对肺癌细胞的凋亡诱导效应 |
| Platycodin-D promotes TRAIL-induced apoptosis by the formation of RIP1-FADD-caspase-8 complex in lung cancer. Zhang Yanwen. Department of Pathology, Central Hospital of Jinhua City, Jinhua, 321000, China |
| 投稿时间：2015-12-26 修订日期：2016-05-20 |
| DOI： |
| 中文关键词: 桔梗皂苷D TRAIL 肺癌 caspase-8 凋亡 |
| 英文关键词:platycodin-D TRAIL lung cancer caspase-8 apoptosis |
| 基金项目: |
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| 中文摘要: |
| 目的: 探讨中药活性成分桔梗皂苷D对抗肿瘤药物TRAIL抗肺癌活性的影响并研究其机制。方法: 用桔梗皂苷D联合TRAIL体外治疗肺癌细胞系A549,MTT法检测肿瘤细胞的细胞活力,Annexin V/PI染色检测肿瘤细胞的凋亡,免疫共沉淀法检测RIP1-FADD-caspase-8复合物的形成,western blot法检测A549细胞caspase-8的活化。结果: 对照组,桔梗皂苷D组,TRAIL组,桔梗皂苷D+TRAIL组对A549细胞活力的抑制率分别为0,(7.6±0.7)%,(13.4±1.0)%,(60.3±4.2)% (P<0.05)。对照组,桔梗皂苷D组,TRAIL组,桔梗皂苷D+TRAIL组对A549细胞凋亡的诱导率分别为(1.6±0.2)%,(4.5±0.3)%,(7.2±0.5)%,(40.2±2.8)% (P<0.05)。免疫共沉淀及western blot结果发现联合桔梗皂苷D后,TRAIL治疗的A549细胞内的RIP1-FADD-caspase-8复合物水平及caspase-8的活化程度显著提高。RIP1 siRNA及z-IETD-fmk能显著抑制桔梗皂苷D对TRAIL的协同作用。对照组,桔梗皂苷D+TRAIL组,桔梗皂苷D+TRAIL+RIP1 siRNA组,桔梗皂苷D+TRAIL+z-IETD-fmk组对A549细胞活力的抑制率分别为0,(61.2±5.0)%,(24.5±1.9)%,(17.5±1.7)% (P<0.05)。对照组,桔梗皂苷D+TRAIL组,桔梗皂苷D+TRAIL+RIP1 siRNA组,桔梗皂苷D+TRAIL+z-IETD-fmk组对A549细胞的凋亡诱导率分别为(1.8±0.2)%,(39.5±2.6)%,(12.3±1.1)%,(9.4±0.8)% (P<0.05)。结论: 桔梗皂苷D通过促进死亡受体复合物的形成增强TRAIL对肺癌细胞的凋亡诱导效应。 |
| 英文摘要: |
| AIM: To investigate the role of platycodin-D in TRAIL-induced cell death in lung cancer. Methods: After the A549 cells were treated with TRAIL combined with platycodin-D, the cell death, cell apoptosis, and the formation of RIP1-FADD-caspase-8 complex were detected by MTT assay, Annexin Ⅴ/PI staining, and co-immunoprecipitation, respectively. In addition, the activation of caspase-8 in A549 cells was evaluated by western blot assay. Results: The cell viability inhibitory rate of A549 cells in control group, platycodin-D group, TRAIL group, platycodin-D+TRAIL group is as follows: 0, (7.6±0.7)%, (13.4±1.0)%, (60.3±4.2)% (P<0.05). The apoptotic ratio of A549 cells in control group, platycodin-D group, TRAIL group, platycodin-D+TRAIL group is as follows: (1.6±0.2)%, (4.5±0.3)%, (7.2±0.5)%, (40.2±2.8)% (P<0.05). The results of co-immunoprecipitation and western blot indicated that the formation of RIP1-FADD-caspase-8 complex and the activation of caspase-8 in TRAIL-treated A549 cells were significantly increased due to the combination of platycodin-D. Treatment of RIP1 siRNA and z-IETD-fmk significantly impaired the synergistic effect of platycodin-D on TRAIL-induced cell death. The cell viability inhibitory rate of A549 cells in control group, platycodin-D+TRAIL group, platycodin-D+TRAIL+RIP1 siRNA group, platycodin-D+TRAIL+z-IETD-fmk group is as follows: 0, (61.2±5.0)%, (24.5±1.9)%, (17.5±1.7)% (P<0.05). The apoptotic rate of A549 cells in control group, platycodin-D+TRAIL group, platycodin-D+TRAIL+RIP1 siRNA group, platycodin-D+TRAIL+z-IETD-fmk group is as follows: (1.8±0.2)%, (39.5±2.6)%, (12.3±1.1)%, (9.4±0.8)% (P<0.05).Conclusion: platycodin-D promotes TRAIL-induced apoptosis by the formation of RIP1-FADD-caspase-8 complex in lung cancer. |
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