| 潘怡宏,王平.补元清脑颗粒对APP /PS1 双转基因小鼠学习记忆及Bax、Bcl-2表达的影响[J].浙江中西医结合杂志,2016,26(11): |
| 补元清脑颗粒对APP /PS1 双转基因小鼠学习记忆及Bax、Bcl-2表达的影响 |
| Effect of Buyuanqingnaokeli on Ability of Learning and Memory and Expression of Bax and Bcl-2 in APP/PS1 Double Transgenic Mice |
| 投稿时间：2016-04-09 修订日期：2016-05-28 |
| DOI： |
| 中文关键词: 补元清脑颗粒 阿尔茨海默病 Morris水迷宫 Western Blot |
| 英文关键词:Buyuanqingnaokeli Alzheimer’s disease Morris Western Blot |
| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 摘要点击次数: 1306 |
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| 中文摘要: |
| 目的：观察补元清脑颗粒对APP/PS1双转基因小鼠学习记忆能力及Bax、Bcl-2表达的影响。方法：将50只APP/PS1双转基因小鼠随机分为模型组、多奈哌齐组、菖蒲益智丸组、补元清脑颗粒高剂量组和低剂量组，每组10只，同月龄相同遗传背景C57BL/6J小鼠10只为空白组。从5月龄开始分别灌胃，每日1次。空白组、模型组均给予等容积生理盐水灌胃。连续灌胃30天后进行Morris水迷宫测试评定各组小鼠学习记忆能力；采用Western Blot法检测小鼠海马组织Bax、Bcl-2蛋白的表达。结果：与模型组比较，补元清脑颗粒高剂量组和低剂量组均可减少小鼠Morris水迷宫测试的上平台潜伏期[(37.90±20.10)s比(66.98±21.00)s，P＜0.01；(47.65±22.93)s比(66.98±21.00)s，P＜0.05]、游泳总路程[(414.94±171.10)cm、(559.72±178.76)cm比(1032.12±118.66)cm，P＜0.01]和第一次抵原平台时间[(21.36±20.22)s、(27.82±21.96)s比(51.71±20.96)s，P＜0.01]，增加穿越平台次数(5.90±1.60比1.80±1.41，P＜0.01；4.60±1.71比1.80±1.41，P＜0.05)和目标象限时间[(17.71±7.58)s、(14.07±7.08)s比(5.55±4.42)s，P＜0.01]，增加小鼠海马组织Bcl-2蛋白相对表达量（0.52±0.07比0.15±0.06，P＜0.01；0.42±0.06比0.15±0.06，P＜0.05）。结论：补元清脑颗粒对APP/PS1 双转基因小鼠的学习记忆能力具有改善作用，且对其具有一定的抗凋亡作用。 |
| 英文摘要: |
| Objective To investigate the effect of Buyuanqingnaokeli on the ability of learning and memory and expression of Bax and Bcl-2 in APP /PS1 double transgenic mice, and to explore the possible therapeutic mechanism. Methods Fifty APP /PS1 double transgenic mice were established and divided into five groups: model group, western medicine group, changpuyizhiwan group, buyuanqingnaokeli ( BYQNKL) high dose group and low dose group. Ten C57BL /6J mice with the same months of age and genetic were blank group. After gavage once a day for 30 days, Morris test, Bax and Bcl-2 of hippocampi of different groups were detected. Results Compared to the model group, the platform latency[(37.90±20.10)s vs (66.98±21.00)s, P＜0.01; (47.65±22.93)s vs (66.98±21.00)s, P＜0.05], total swimming distance[(414.94±171.10)cm, (559.72±178.76)cm vs (1032.12±118.66)cm, P＜0.01] and first time reach for the original platform[(21.36±20.22)s, (27.82±21.96)s vs (51.71±20.96)s, P＜0.01] of BYQNKL high dose group and low dose group were reduced, the target quadrant time[(17.71±7.58)s, (14.07±7.08)s vs (5.55±4.42)s, P＜0.01], through platform number(5.90±1.60 vs 1.80±1.41, P＜0.01; 4.60±1.71 vs 1.80±1.41, P＜0.05) and Bcl-2 relative transcript level(0.52±0.07 vs 0.15±0.06, P＜0.01; 0.42±0.06 vs 0.15±0.06, P＜0.05) of BYQNKL high dose group and low dose group were increased. Conclusion BYQNKL has learning and memory improvement and anti-apoptosis effects of APP /PS1 double transgenic mice． |
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