| 许雷来,谢小红.华蟾素注射液对乳腺癌MDA-MB-231细胞E-cad、N-cad表达的影响[J].浙江中西医结合杂志,2017,27(2): |
| 华蟾素注射液对乳腺癌MDA-MB-231细胞E-cad、N-cad表达的影响 |
| Effect of Cinobufotalin Injection on the Expressions of E-cad, N-cad in breast cancer a Cell Line MDA-MB-231. |
| 投稿时间：2016-08-28 修订日期：2016-11-12 |
| DOI： |
| 中文关键词: 华蟾素注射液 乳腺癌 MDA-MB-231细胞 E-cad/N-cad |
| 英文关键词:Cinobufotalin Injection Breast cancer Cell line MDA-MB-231 E-cad/N-cad |
| 基金项目:浙江省自然科学基金资助项目（No.LY12H16008)；浙江省大学生科技创新活动计划暨新苗人才计划（No.2015R410026） |
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| 中文摘要: |
| 目的：通过观察华蟾素注射液对乳腺癌MDA-MB-231细胞E-cad、N-cad表达的影响，探讨华蟾素注射液对乳腺癌细胞上皮间质转化（EMT）的影响并初步探究华蟾素注射液抑制乳腺癌转移机制。方法：采用四氮唑蓝（MTT）比色法观察不同浓度华蟾素注射液（1:5、1:10、1:20、1:40、1:80、1:160、1:320稀释）对MDA-MB-231细胞增殖影响；实时荧光定量PCR（qRT-PCR）检测法检测华蟾素注射液处理MDA-MB-231细胞后，细胞中E-cad/N-cad mRNA表达水平；蛋白印迹法（Westernblot）检测华蟾素注射液处理MDA-MB-231细胞后，细胞中E-cad/N-cad蛋白表达水平。结果：高浓度华蟾素注射液48h对MDA-MB-231细胞抑制率较中剂量、低剂量组更加明显，差异具有统计学意义（P<0.05），实时荧光定量PCR结果显示华蟾素注射液可以下调MDA-MB-231细胞N-cad的表达和上调E-cad表达，与空白组相比，差异具有统计学意义(P<0.01)。Westernblot结果显示华蟾素注射液能降低N-cad蛋白的表达和上调E-cad蛋白的表达，与空白组相比，差异具有统计学意义(P<0.05)。结论：华蟾素能通过上调E-cad的表达和下调N-cad的表达，抑制MDA-MB-231细胞的上皮间质转化（EMT）转变，从而降低乳腺癌MDA-MB-231细胞的转移能力。 |
| 英文摘要: |
| [Purpose]To investigate the effect of Cinobufotalin Injection on the expressions of E-cad, N-cad in breast cancer a Cell Line MDA-MB-231, in order to explore the inhibiting effect of Cinobufotalin Injection on the metastasis of breast cancer.[Methods] Different concentrations of Cinobufotalin Injection were prepared（1:5、1:10、1:20、1:40、1:80、1:160、1:320 diluted）, and proliferation effects on cell line lovo were observed by MTT.The expressions of E-cad and N-cad mRNA were detected by qRT-PCR, and the expression of E-cad and N-cad protein was detected by Western Blot assay.[Results] The high concentration group of Cinobufotalin Injection had a higher cell inhibition effect at 48 hours, compared with the medium group and low group (P<0.05).The expressions of N-cad in drug group were down-regulated and the expression of E-cad was up-regulated,Compared with the blank and normal control group by qRT-PCR (P<0.01) and Western Blot assay (P<0.05).[Conclusion]Cinobufotalin Injection can inhibit the EMT transition of cell line MDA-MB-231 and decrease its migration and invasive ability through the down-regulation of N-cad expressions and the up-regulation of E-cad expression. |
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