| 黄玲燕.白藜芦醇增强乳腺癌细胞对γδ T细胞的敏感性及其机制研究[J].浙江中西医结合杂志,2017,27(8): |
| 白藜芦醇增强乳腺癌细胞对γδ T细胞的敏感性及其机制研究 |
| The effect and mechanism of resveratrol on increasing the sensitivity of breast cancer cells to gd T cells |
| 投稿时间：2017-03-15 修订日期：2017-05-04 |
| DOI： |
| 中文关键词: 白藜芦醇 c-FLIP γδ T细胞 乳腺癌 |
| 英文关键词:resveratrol c-FLIP γδ T cells breast cancer |
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| 中文摘要: |
| 目的: 探讨gd T细胞对乳腺癌细胞的杀伤活性并研究白藜芦醇是否能增强gd T细胞的抗肿瘤活性。方法：体外扩增培养人gd T细胞并用流式细胞术进行鉴定。实验分为对照组,白藜芦醇组,gd T细胞组,白藜芦醇+gd T细胞组及白藜芦醇+gd T细胞+c-FLIP质粒组,LDH释放实验检测gd T细胞对MDA-MB-231的杀伤活性；western blot实验检测白藜芦醇及gd T细胞处理后MDA-MB-231细胞c-FLIP的表达水平,caspase 8、caspase 9、caspase 3的活化和细胞色素c的释放。结果：流式细胞实验结果显示gd T细胞能通过体外扩增获得。LDH释放实验显示gd T细胞+白藜芦醇组MDA-MB-231的LDH释放率(68.2±7.1)显著高于gd T细胞组(21.4±3.2, P<0.05)和白藜芦醇+gd T细胞+c-FLIP质粒组(27.9±3.6, P<0.05)。western blot实验结果显示白藜芦醇处理能显著降低MDA-MB-231细胞中c-FLIP蛋白的表达。另外,western blot实验结果也显示gd T细胞+白藜芦醇组MDA-MB-231的活化caspase 8 、活化Bid及细胞色素c的释放均显著高于gd T细胞组和白藜芦醇+gd T细胞+c-FLIP质粒组。结论：白藜芦醇下调乳腺癌细胞c-FLIP的表达提高乳腺癌细胞对gd T细胞的敏感性。 |
| 英文摘要: |
| AIM: To investigate the effect and mechanism of resveratrol on enhancing the cytotoxicity of gd T cells to breast cancer. Methods: Amplifying the gd T cells in vitro. Cell population of gd T was detected by flow cytometry. To perform the following experiments, MDA-MB-231 cells were divided into control group, resveratrol group, gd T cells group, resveratrol + gd T cells group and resveratrol + gd T cells + c-FLIP plasmid group. LDH release assays were performed to evaluate the cytotoxicity of gd T cells to MDA-MB-231. Western blot analysis was performed to evaluate the expression of c-FLIP, activation of caspase 8, caspase 9, caspase 3, Bid and release of cytochrome c in MDA-MB-231 cells. Results: Flow cytometry analysis showed that gd T was successfully obtained. Results of LDH release assays showed that the LDH release rate in gd T cells + resveratrol group (68.2±7.1) was significantly higher than the gd T cells group (21.4±3.2, P<0.05) and gd T cells + resveratrol + c-FLIP plasmid group (27.9±3.6, P<0.05). Results of western blot assays showed that resveratrol downregulated the expression of c-FLIP. In addition, results of western blot assays also showed that activation of caspase 8, caspase 9, caspase 3, Bid and release of cytochrome c was significantly higher than that in the gd T cells group and gd T cells + resveratrol + c-FLIP plasmid group. Conclusion: Resveratrol increased the sensitivity of breast cancer cells to gd T cells through inhibition of c-FLIP expression. |
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