| 叶茂盛,赵东杰,周勇,蒋蓝英.人参皂苷Rg1对β淀粉样蛋白诱导的神经细胞凋亡的研究[J].浙江中西医结合杂志,2017,27(8): |
| 人参皂苷Rg1对β淀粉样蛋白诱导的神经细胞凋亡的研究 |
| Effect of ginsenoside Rg1 on β-amyloid induced neuron apoptosis |
| 投稿时间：2017-04-05 修订日期：2017-05-03 |
| DOI： |
| 中文关键词: 阿尔茨海默病 人参皂苷 β-淀粉样蛋白 细胞凋亡 |
| 英文关键词:Alzheimer’s disease ginsenoside β-amyloid peptide apoptosis |
| 基金项目:浙江省中医药科技计划项目(2010ZB112) |
|
| 摘要点击次数: 1111 |
| 全文下载次数: 20 |
| 中文摘要: |
| 目的 探讨人参皂苷Rg1对大鼠海马组织β淀粉样蛋白（Aβ）诱导的神经细胞凋亡的影响。方法 将36只SD雄性大鼠分为3组：假手术组、Aβ模型组和人参皂苷Rg1干预组。Aβ模型组和人参皂苷干预组组直接注射β淀粉样蛋白1-42(Aβ1-42)于大鼠海马组织；人参皂苷干预组用人参皂苷Rg1给予大鼠连续灌胃30 d。采用Morris水迷宫测试大鼠学习记忆能力，采用甲醇刚果红染色观察人参皂苷Rg1对海马组织Aβ的沉积影响，采用TUNEL法检测海马神经细胞凋亡。结果 假手术组大鼠水迷宫测试逃避潜伏时间为（47.36±12.21）s，穿越平台次数为(7.48±1.25)次。Aβ模型组大鼠水迷宫测试逃避潜伏时间为（84.52±16.68）s，穿越平台次数为(2.57±0.64)次。与Aβ模型组比较，人参皂苷干预组大鼠的逃避潜伏时间[（63.22±12.05）s]和穿越平台次数[(5.22±1.31)次]均得到显著的改善(P<0.05)。Aβ模型组大鼠海马组织出现明显Aβ沉积；人参皂苷干预组大鼠海马组织阳性斑块数量少于Aβ模型组。Aβ模型组海马组织细胞出现明显的细胞凋亡，人参皂苷干预组海马组织TUNEL阳性神经细胞数目[（73.20±1.71）个]比Ａβ模型组[（101.83±1.45）个]明显减少(P<0.01)。结论 人参皂苷Rg1可明显改善Aβ所致AD大鼠学习记忆功能，减少海马组织的Aβ沉积，抑制大鼠海马Aβ诱导的细胞凋亡。 |
| 英文摘要: |
| Objective: To investigate the effect of ginsenoside Rg1 on β-amyloid induced neuron apoptosis in the hippocampus area. Methods: Thirty six Sprague-Dawley rats were assigned into 3 groups. Rats in the sham-operated group were injected with normal saline. Rats in the Aβ group and ginsenoside Rg1 group were injected with Aβ1-42. Rats in the ginsenoside Rg1 group were feeded with ginsenoside Rg1 solution for 30 days consecutively. The Morris water maze was used to investigate the ability of learning and memory in the rats. The effect of Aβ and ginsenoside Rg1 on the hippocampus cells was observed by Congo red staining of methanol.The apoptotic neurons were detected by TUNEL method.Results: The escape latency of rats was （47.36±12.21）s and and the number of rats crossing platform was (7.48±1.25) in the sham-operated group.The escape latency of rats was （84.52±16.68）s and and the number of rats crossing platform was (2.57±0.64) in the Aβ group.Compared with Aβ group,the escape latency [（63.22±12.05）s] of rats was significantly decreased(P<0.05)，and the number of rats crossing platform [(5.22±1.31)] was significantly increased in the ginsenoside Rg1 group (P<0.05)．The β-amyloid peptide deposition was found in hippocampus of the rats.More positive staining materials aggradated in the Aβ group compared with the ginsenoside Rg1 group by Congo red staining.The apoptotic neurons in hippocampus of the rats in Aβ injection group were increased more significantly than that in ginsenoside Rg1 group and sham-operated group.The number of TUNEL positive neuron in hippocampus of ginsenoside Rg1 group（73.20±1.71）is obviously lower than the Aβ group（101.83±1.45）, the difference between the two groups is statistically significant(P<0.01).Conclusion: Ginsenoside Rg1 can reduce the deposition of Aβ in the hippocampus and improve the learning and memory function of rats. Ginsenoside Rg1 can reduce the Aβ-induced apoptosis in the hippocampus. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
