| 叶海南.川楝素通过XIAP途径增强肺癌细胞对顺铂的敏感性[J].浙江中西医结合杂志,2018,28(4): |
| 川楝素通过XIAP途径增强肺癌细胞对顺铂的敏感性 |
| Toosendanin increased the sensitivity of lung cancer cells to cisplatin through XIAP pathway |
| 投稿时间：2017-05-15 修订日期：2018-01-05 |
| DOI： |
| 中文关键词: 川楝素 XIAP 顺铂 肺癌 |
| 英文关键词:toosendanin XIAP cisplatin lung cancer |
| 基金项目: |
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| 中文摘要: |
| 目的: 研究天然药物活性成分川楝素对顺铂抗肺癌活性的协同效应并研究其机制。方法：实验对象为人肺癌细胞系A549,实验分为对照组,川楝素组,顺铂组,川楝素+顺铂组及川楝素+顺铂+XIAP质粒。MTT实验检测各组A549的细胞活力抑制率；流式细胞实验检测各组A549的细胞的凋亡；免疫共沉淀及western blot实验检测各组A549的细胞中XIAP表达水平及其与caspase-9和caspase-3的相互作用；western blot实验检测各组A549的细胞caspase-9和caspase-3的活化。结果：顺铂+川楝素组A549的细胞活力抑制率(66.8±5.9)显著高于顺铂单治疗组(15.2±1.2, P<0.05)和川楝素+顺铂+XIAP质粒组(20.4±1.8, P<0.05)；顺铂+川楝素组A549的细胞凋亡率(34.5±2.6)显著高于顺铂单治疗组(9.7±0.8, P<0.05)和川楝素+顺铂+XIAP质粒组(13.3±1.2, P<0.05)；免疫共沉淀及western blot实验显示川楝素能显著抑制A549细胞中XIAP的表达及其与caspase-9和caspase-3的相互作用,川楝素促进顺铂对caspase-9和caspase-3的活化。结论：川楝素通过下调XIAP的表达增强肺癌细胞对顺铂的敏感性。 |
| 英文摘要: |
| AIM: To investigate the synergistic effect and mechanism of toosendanin on enhancing the cytotoxicity of cisplatin to lung cancer. Methods: Human lung cancer cell line A549 was used as the experimental subject. Cells were divided into control group, toosendanin group, cisplatin group, toosendanin + cisplatin group and toosendanin + cisplatin + XIAP plasmid group. MTT assays were performed to evaluate the inhibitory rate of cell viability in each group. Flow cytometry analysis was performed to detect the cell apoptosis of A549 cells in each group. Co-immunoprecipitation and western blot analysis were performed to evaluate the expression of XIAP and interaction with XIAP, caspase-9 and caspase-3. Western blot analysis was performed to detect the activation of caspase-9 and caspase-3. Results: Inhibitory rate of cell viability in cisplatin+ toosendanin group (66.8±5.9) was significantly higher than the cisplatin group (15.2±1.2, P<0.05) and cisplatin + toosendanin + XIAP plasmid group (20.4±1.8, P<0.05). Apoptotic rate of A549 cells in cisplatin + toosendanin group (34.5±2.6) was significantly higher than that in the cisplatin group (9.7±0.8, P<0.05) and cisplatin + toosendanin + XIAP plasmid group (13.3±1.2, P<0.05). Results of co-immunoprecipitation and western blot analysis showed that toosendanin suppressed the expression of XIAP and interaction with XIAP and caspase-9 and caspase-3. In addition, results of western blot analysis showed that toosendanin promoted the activation of caspase-9 and caspase-3 induced by cisplatin. Conclusion: Toosendanin increased the sensitivity of lung cancer cells to cisplatin through downregulating the expression of XIAP |
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