| 戴卓睿,毛其芬.二氢青蒿素抑制ATF2的磷酸化提高胃癌细胞对5-氟尿嘧啶的敏感性[J].浙江中西医结合杂志,2018,28(5): |
| 二氢青蒿素抑制ATF2的磷酸化提高胃癌细胞对5-氟尿嘧啶的敏感性 |
| Dihydroartemisinin increases sensitivity of gastric cancer cells to 5-fluorouracil through suppression of ATF2 phosphorylation |
| 投稿时间：2017-11-09 修订日期：2018-03-23 |
| DOI： |
| 中文关键词: 二氢青蒿素 5-氟尿嘧啶 胃癌 ATF2 凋亡 |
| 英文关键词:Dihydroartemisinin 5-fluorouracil gastric cancer ATF2 apoptosis |
| 基金项目: |
|
| 摘要点击次数: 1368 |
| 全文下载次数: 16 |
| 中文摘要: |
| 目的: 探讨中药活性成分二氢青蒿素是否对5-氟尿嘧啶有协同抗胃癌效应并研究其机制。方法: MTT实验检测胃癌细胞系BGC-823的细胞活力；流式细胞术检测BGC-823细胞的凋亡；western blot实验检测BGC-823细胞中ATF2、磷酸化ATF2、Bcl-xl的表达水平及细胞色素c的释放水平和caspase-3的活化水平。结果: 5-氟尿嘧啶联合二氢青蒿素对BGC-823的细胞活力抑制率(67.1±5.5)和凋亡诱导率(37.3±2.9)显著高于5-氟尿嘧啶单治疗组的细胞活力抑制率(18.9±1.5,P<0.05)和凋亡诱导率(9.5±0.9,P<0.05)。5-氟尿嘧啶联合二氢青蒿素治疗组的ATF2磷酸化水平和Bcl-xl表达水平均显著低于5-氟尿嘧啶单治疗组,同时5-氟尿嘧啶联合二氢青蒿素治疗组的细胞色素c释放水平及caspase-3活化水平均显著高于5-氟尿嘧啶单治疗组。另外,5-氟尿嘧啶+二氢青蒿素+ATF2质粒组BGC-823的细胞活力抑制率(26.5±2.1)和凋亡诱导率(14.9±1.1)显著低于5-氟尿嘧啶联合二氢青蒿素组BGC-823的细胞活力抑制率(67.1±5.5,P<0.05)和凋亡诱导率(37.3±2.9,P<0.05)。结论: 二氢青蒿素通过抑制ATF2的磷酸化增强5-氟尿嘧啶对胃癌细胞的杀伤活性。 |
| 英文摘要: |
| AIM: To investigate the synergistic effect and mechanism of dihydroartemisinin on increasing the cytotoxicity of 5-fluorouracil to gastric cancer. Methods: MTT assay was performed to test the cell viability of BGC-823. Flow cytometry analysis was performed to detect the apoptosis of BGC-823 cells. Western blot analysis was performed to evaluate the expression of ATF2, phosphorylated ATF2 and Bcl-xl, and release of cytochrome c, and the activation of caspase-3 in BGC-823 cells. Results: Cell viability inhibitory rate (67.1±5.5) and apoptotic rate (37.3±2.9) of BGC-823 cells in 5-fluorouracil plus Dihydroartemisinin group was significantly higher than the cell viability inhibitory rate (18.9±1.5, P<0.05) and apoptotic rate (9.5±0.9, P<0.05) of BGC-823 cells in 5-fluorouracil single treatment group. Phosphorylation of ATF and expression of Bcl-xl in the 5-fluorouracil plus dihydroartemisinin group was significantly lower than that in the 5-fluorouracil group. Meanwhile, the release of cytochrome c and activation of caspase-3 in the 5-fluorouracil plus dihydroartemisinin group was significantly higher than that in the 5-fluorouracil single treatment group. In addition, cell viability inhibitory rate (26.5±2.1) and apoptotic rate (14.9±1.1) of BGC-823 in 5-fluorouracil + dihydroartemisinin + ATF2 plasmid group was significantly lower than the cell viability inhibitory rate (67.1±5.5, P<0.05) and apoptotic rate (37.3±2.9, P<0.05) of BGC-823 in 5-fluorouracil + dihydroartemisinin group. Conclusion: Dihydroartemisinin enhanced the cytotoxicity of 5-fluorouracil against gastric cancer through suppression of ATF2 phosphorylation. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
