| 饶春晖,杨燕,陆淼炯.原儿茶酸对金黄色葡萄球菌性肺炎小鼠模型的保护作用及相关机制研究[J].浙江中西医结合杂志,2018,28(11): |
| 原儿茶酸对金黄色葡萄球菌性肺炎小鼠模型的保护作用及相关机制研究 |
| The protective effect of Protocatechuic Acid on Staphylococcus aureus pneumonia and the associated immunological mechanisms in mice model |
| 投稿时间：2018-03-13 修订日期：2018-10-10 |
| DOI： |
| 中文关键词: 原儿茶酸 金黄色葡萄球菌 肺炎 P65蛋白 |
| 英文关键词:Protocatechuic Acid Staphylococcus aureus pneumonia P65 |
| 基金项目:蛇六谷提取物介导内质网应激逆转肝癌5-FU耐药作用研究 浙江省中医药管理局基金（2018ZB088） |
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| 中文摘要: |
| 【摘要】 目的 研究原儿茶酸（Protocatechuic Acid, PA） 对金黄色葡萄球菌（Staphylococcus aureus, SA）性小鼠肺炎（pneumonia）的保护作用，并探讨其相关机制。方法 将小鼠和RAW264.7细胞随机分为对照组、SA模型组和原儿茶酸预处理组，通过给小鼠行气道滴注构建SA肺炎模型和体外巨噬细胞RAW264.7感染模型，模型构建前1h行PA预处理。采用苏木精伊红染色方法（hematoxylin-eosin staining, HE）检测各组小鼠肺组织病理变化，Western Blot检测RAW264.7细胞中P38MAPK和p-P38MAPK、ERK MAPK和p-ERK MAPK、JNK MAPK和p-JNK MAPK、P65和p-P65蛋白的表达情况，采用实时荧光定量PCR法（RT-qPCR）和酶联免疫吸附法（ELISA）测定肿瘤坏死因子-α（tumor necrosis factor-α, TNF-α）和白介素-6（Interleukin-6, IL-6）的表达，这些指标用于评估PA对SA肺炎的作用。结果 动物实验中，与模型组相比，PA预处理可减轻SA肺炎，减轻肺组织病理改变，显著性降低肺组织和肺泡灌洗液中炎症因子TNF-α和IL-6表达（均为P < 0.05）；细胞实验中，与模型组相比，PA预处理可显著降低细胞上清液和细胞中炎症因子TNF-α和IL-6表达（均为P < 0.05），并且抑制P65蛋白的活化，但其对MAPK相关蛋白的表达无影响。结论 原儿茶酸对SA肺炎具有保护作用，相关保护作用机制可能与抑制P65炎症信号通路的活化、降低炎症因子的表达有关。 |
| 英文摘要: |
| 【Abstract】 Objective To explore the role of Protocatechuic Acid in the treatment of Staphylococcus aureus pneumonia in mice and the possible mechanisms．Methods The mice and the cells were randomly divided into three groups: normal control group, SA model group and PA pretreatment group, The SA pneumonia was established by intratracheal instillation of SA, and the RAW264.7 infection model was stimulated by the moi of 10. The pathological changes of lung tissues were observed by HE staining, the level of TNF-α and IL-6 in mice and cells were tested by ELISA and RT-qPCR, the expressions of P38MAPK、p-P38MAPK、ERK MAPK、p-ERK MAPK、JNK MAPK、p-JNK MAPK、P65 and p-P65 were detected by Western Blotting. All the results were measured to evaluate the effect of PA on SA pneumonia．Results In vivo, compared with the model group, Pretreatment with PA could significantly attenuate lung injury, improve the pathological changes of lung tissues, the expression of TNF-α and IL-6 in lung tissues were significantly increased (all P <0.05). In vitro, compared with the model group, the expression of TNF-α and IL-6 in cells were significantly increased in PA pretreatment group (all P <0.05), the expressions of p-P65 in cells were markedly suppressed in PA pretreatment group, without influencing those of p-P38、p-ERK and p-JNK．Conclusion PA has remarkable protective effect on SA pneumonia, the mechanism is possibly related to the inhibition P65 activation and reduction of inflammatory response. |
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