| 刘芳,魏威,刘承浩,汪存信,叶丽红,刘敏.激光针刀对椎动脉型颈椎病模型兔椎动脉磷脂酰肌醇3-激酶mRNA表达的影响[J].浙江中西医结合杂志,2019,29(3): |
| 激光针刀对椎动脉型颈椎病模型兔椎动脉磷脂酰肌醇3-激酶mRNA表达的影响 |
| Influence of laser needle-knife on PI-3K mRNA expression in cervical spondylotic arteriopathy model rabbits |
| 投稿时间：2018-10-29 修订日期：2018-11-09 |
| DOI： |
| 中文关键词: 激光针刀 椎动脉型颈椎病 PI-3K mRNA表达 |
| 英文关键词:laser-needle knife cervical vertebral artery disease PI-3K mRNA expression |
| 基金项目:国家自然科学基金青年基金(NO.81704144)；浙江省公益技术应用研究计划(NO.2016C37136) |
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| 中文摘要: |
| 目的：探讨激光针刀对CSA(椎动脉型颈椎病)模型兔椎动脉PI-3K(磷脂酰肌醇3-激酶) mRNA表达的影响,明确其初步治疗机理。方法：建立CSA家兔模型,将造模成功的30 只CSA兔用随机数字表法分为模型组、针刺组和激光针刀组, 每组10 只,另取10 只正常家兔为正常组。对针刺组兔给予针刺“风池穴”、“颈夹脊穴”治疗,对激光针刀组兔给予第五颈椎棘突旁夹脊穴激光针刀治疗,模型组和正常组仅给予相同的固定,实验前后观察家兔行为学体征,分别于造模前1天、造模后第14天、24天、34天称取体重。采用real-time PCR(实时定量荧光PCR)技术检测各组兔椎动脉血管PI-3K mRNA表达情况。结果：治疗后针刺组和激光针刀组兔行为学体征有不同程度的改善,与模型组比较,针刺组、激光针刀组兔体重明显高于模型组(分别为P<0.05、P<0.01)。激光针刀组和针刺组兔颈动脉PI-3KmRNA表达明显高于模型组(分别为P<0.01、P<0.05),针刺组与激光针刀组比较,无显著性差异(P>0.05)。结论：激光针刀能有效干预CSA模型兔椎动脉的PI-3KmRNA表达。 |
| 英文摘要: |
| ABSTRACT: Objective：To determine the effect of laser needle-knife on PI-3K
mRNA expression of vertebral arteries in a rabbit model of cervical spondylotic arteriopathy (CSA) and the mechanism of action involved. Methods: Forty healthy general-grade rabbits were divided into a normal control group, model group, acupuncture group, and laser needle-knife group (n=10 rabbits per group), and the CSA rabbit model was established in all but groups but the normal control group. CSA model rabbits in the acupuncture group were treated by acupuncture at the “fengchi” and “cervical jiaji” points, whereas rabbits in the laser needle-knife group were treated with laser needle-knife targeting the “jiaji” points near the C5 spinous process. Rabbits in the normal control and model groups were fixed using similar methods. Behavioral characteristics of all rabbits were evaluated before and after treatment. PI-3K mRNA expression in vertebral arteries were determined by real-time PCR. Results: The behavioral signs of rabbits were improved after treatment in both the acupuncture and laser needle-knife groups. The weight of rabbits in laser needle-knife and acupuncture group was significantly higher than that in control group (P<0.05、P<0.01).PI-3K mRNA expression in laser needle-knife and acupuncture group was significantly higher than that in control group (P<0.01、P<0.05). No significant differences were found in PI-3K mRNA expression levels among acupuncture and laser needle-knife groups (P>0.05). Conclusion: Laser needle-knife could effectively intervene the mRNA expression of PI-3K. |
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