| 陈珍,俞颂平,李俊,兰淑琴.七叶皂苷钠对糖尿病大鼠血-视网膜屏障损伤的保护作用及其机制[J].浙江中西医结合杂志,2019,29(7): |
| 七叶皂苷钠对糖尿病大鼠血-视网膜屏障损伤的保护作用及其机制 |
| The Protective Effects of ESC in Blood-Retinal Barrier Damage and the Mechanism |
| 投稿时间：2018-11-11 修订日期：2019-03-16 |
| DOI： |
| 中文关键词: 七叶皂苷钠 糖尿病视网膜病变 血-视网膜屏障 ROCK |
| 英文关键词:: Esc diabetic retinopathy blood-retinal barrier ROCK |
| 基金项目: |
|
| 摘要点击次数: 1327 |
| 全文下载次数: 10 |
| 中文摘要: |
| 目的明确七叶皂苷钠（ESC）对糖尿病大鼠血-视网膜屏障(BRB)的保护作用，进一步探讨其作用机制。方法采用链脲佐菌素（STZ）诱导SD（Sprague Dawley）大鼠建立糖尿病性视网膜病变（DR）模型，予以七叶皂苷钠（1.5mg/kg?d）灌胃处理，通过检测视网膜中伊文思蓝（EB）渗漏量评估ESC对DR大鼠BRB的影响；采用Western blot法检测视网膜组织Occludin蛋白、Rho相关卷曲螺旋形成蛋白激酶（Rho associated coiledcoil forming protein kinase,ROCK）蛋白及磷酸化肌球蛋白轻链（P-MLC）蛋白表达的量。结果ESC能够减少DR大鼠的BRB渗漏情况（P < 0.01），ESC能够增加Occludin蛋白表达（P < 0.01），同时抑制ROCK/P-MLC通路的激活（P < 0.01）。结论七叶皂苷钠对糖尿病大鼠BRB具保护作用，其机制为增加DR大鼠视网膜组织Occludin蛋白的表达，同时抑制炎性信号通路的激活。 |
| 英文摘要: |
| Objective:To testify the protection effects of ESC on blood-retinal barrier (BRB) in diabetic rats, and investigate its mechanism.Methods:DR modelwas induced by STZ injection in SDrats and then the DR rats were given with 15 mg/kg?dESC through the stomach. The effect of ESC to BRB was evaluated by the expression of Evansblue(EB) leakage in retial;Thelevles of Occludin,ROCK and its substrate P-MLCin retialwere detected by Western blotting. Results:TheEB leakage through BRBwas reduced by ESC(P < 0.01)which increase the expression of Occludin(P < 0.01)and also inhibitethe activation of ROCK pathway(P < 0.01).Conclusion:ESCcan protect BRB of DR rats by increasing the expression of Occludinin retial of DR rats and inhibiting the activation of the inflammatory signaling pathway. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
