| 沈云飞,詹建伟.苦参对耐吉非替尼人肺腺癌PC9/ZD细胞上皮间质转化及吉非替尼药物敏感性的影响[J].浙江中西医结合杂志,2019,29(9): |
| 苦参对耐吉非替尼人肺腺癌PC9/ZD细胞上皮间质转化及吉非替尼药物敏感性的影响 |
| Related Clinical Analysis on the Influence of Matrine and Gefitinib on Epithelial Interstitial Transformation of Lung Adenocarcinoma under Different Conditions. |
| 投稿时间：2019-01-21 修订日期：2019-08-16 |
| DOI： |
| 中文关键词: 苦参 吉非替尼 肺腺癌 上皮间质转化 耐药 |
| 英文关键词:Radix Sophorae Flavescentis gefitinib lung adenocarcinoma epithelial mesenchymal transition drug resistance |
| 基金项目:浙江省中医药科技计划项目(2015ZB080) |
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| 中文摘要: |
| 目的 明确苦参对耐吉非替尼人肺腺癌PC9/ZD细胞上皮间质转化及吉非替尼药物敏感性的影响。方法 选取对细胞无毒性作用的苦参浓度,采用体外苦参作用于耐吉非替尼人肺腺癌PC9/ZD细胞；分为对照组,吉非替尼组,苦参处理加吉非替尼组。分别通过MTT法、transwell实验检测经不同组细胞株的增殖侵袭转移能力的变化；通过Western blot实验检测波形蛋白、上皮钙粘附分子在不同组表达差异,流式细胞术测细胞凋亡相差异情况。结果 当苦参浓度为0.5mg/ml时24h细胞生长抑制率小于10%,对细胞无毒性作用,经此浓度苦参作用可以增强吉非替尼诱导的PC9/ZD细胞波形蛋白表达下调,E钙粘附蛋白达上调,N钙粘附蛋白表达下调,增强吉非替尼对细胞侵袭能力的抑制,同时使得细胞对吉非替尼诱导的细胞增殖抑制和细胞调亡的敏感性明显增强,结论 苦参可逆转人肺腺癌PC9/ZD细胞上皮间质转化,增加对吉非替尼药物敏感性。 |
| 英文摘要: |
| Objective To investigate the effect of Sophora flavescens on epithelial-mesenchymal transition and gefitinib sensitivity of gefitinib-resistant human lung adenocarcinoma PC9 / ZD cells. Method Sophora flavescens was used to treat PC9 / ZD cells of human lung adenocarcinoma resistant to gefitinib in vitro. The cells were divided into control group, gefitinib group and Sophora flavescens plus gefitinib group. MTT assay and Transwell assay were used to detect the proliferation, invasion and metastasis of different groups of cell lines. Western blot assay was used to detect the expression of vimentin and epithelial calcium adhesion molecule in different groups, and flow cytometry was used to detect the difference of apoptosis. Results When the concentration of Sophora flavescens was 0.5mg/ml, the inhibition rate of cell growth was less than 10% at 24 h. The effect of Sophora flavescens could enhance the down-regulation of vimentin expression, up-regulation of E-cadherin, down-regulation of N-cadherin expression and enhancement of invasion ability of gefitinib on PC9/ZD cells. Inhibiting the proliferation of cells induced by gefitinib and increasing the sensitivity of cells to apoptosis. Conclusion Sophora flavescens can reverse the epithelial-mesenchymal transformation of human lung adenocarcinoma PC9 / ZD cells and increase the sensitivity to gefitinib. |
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