| 卢裕强,郭汝宝.推拿手法对家兔骨骼肌失神经支配后成肌调节因子Myf-5、Myogenin表达的影响[J].浙江中西医结合杂志,2019,29(12): |
| 推拿手法对家兔骨骼肌失神经支配后成肌调节因子Myf-5、Myogenin表达的影响 |
| Influence of Tuina Manipulation on Myogenic regulatory factor Myf-5 and Myogenin Induced by Peripheral Nerve Injury |
| 投稿时间：2019-06-12 修订日期：2019-09-21 |
| DOI： |
| 中文关键词: 骨骼肌失神经支配 推拿手法 肌湿重比 成肌调节因子 |
| 英文关键词:Denervated skeletal muscle Tuina therapy Gastrocnemius weight ratio Myogenic regulatory factor |
| 基金项目:国家自然科学(81303064,81873391),国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 中文摘要: |
| 目的：通过观察推拿手法对家兔失神经支配骨骼肌干预后肌湿重、成肌调节因子中生肌因子5(Myogenic factor 5,Myf-5)、肌细胞生成素(Myogenin)表达的变化，探讨推拿手法对失神经支配骨骼肌的影响。方法：新西兰家兔随机分为4大组（n=30）：正常对照组、模型对照组、神经生长因子(Nerve growth factor,NGF)治疗组、手法治疗组，每大组再随机分为5个亚组（n=6）：2w组、3w组、1m组、2m组、4m组。分别干预后处死取材，称量肌湿重，计算术侧与健侧的肌湿重比值；检测Myf-5信使核糖核酸(mRNA)、Myogenin mRNA表达水平。结果：①腓肠肌肌湿重比：NGF治疗组在2w（0.79±0.03）、3w（0.70±0.06）、1m（0.58±0.04）、2m（0.44±0.05），手法治疗组在2w（0.72±0.05）、3w（0.55±0.04）、1m（0.55±0.09）、2m（0.49±0.03）、4m（0.50±0.03）高于模型观察组2w（0.53±0.01）、3w（0.41±0.03）、1m（0.40±0.03）、2m（0.38±0.05）、4m（0.38±0.05），差异有统计学意义（P＜0.05）。手法治疗组在2w（0.72±0.05）、3w（0.55±0.04）低于NGF治疗组2w（0.79±0.03）、3w（0.70±0.06），4m（0.50±0.03）时则高于NGF治疗组（0.42±0.02），差异有统计学意义（P＜0.05）。②腓肠肌Myf-5 mRNA表达：NGF治疗组在2w（18.50±1.79）、3w（12.52±0.58）、1m（5.30±1.76）、2m（2.82±0.23），手法治疗组在2w（20.48±2.20）低于模型观察组2w（40.91±10.31）、3w（25.25±1.27）、1m（13.91±1.40）、2m（8.83±0.86），手法治疗组在1m（16.50±1.17）、2m（12.20±0.84）、4m（5.32±0.60）高于模型观察组1m（13.91±1.40）、2m（8.83±0.86）、4m（3.82±0.54），差异有统计学意义（P＜0.05）。手法治疗组在3w（37.21±9.10）、1m（16.50±1.17）、2m（12.20±0.84）、4m（5.32±0.60）时高于NGF治疗组3w（12.52±0.58）、1m（5.30±1.76）、2m（2.82±0.23）、4m（3.88±0.57），差异有统计学意义（P＜0.05）。③腓肠肌Myogenin mRNA表达：NGF治疗组在2w（94.31±23.87）、3w（165.95±29.58）、1m（156.59±64.76）、4m（4.05±0.51），手法治疗组在4m（5.74±1.35）低于模型观察组2w（185.92±48.71）、3w（359.21±116.88）、1m（597.50±146.66）、4m（63.13±12.24），手法治疗组在2m（1026.75±132.63）高于模型观察组（168.84±31.85），差异有统计学意义（P＜0.05）。手法治疗组在2w（209.80±91.25）、3w（482.37±97.95）、1m（667.73±97.95）、2m（1026.75±132.63）均高于NGF治疗组2w（94.31±23.87）、3w（165.95±29.58）、1m（156.59±64.76）、2m（140.63±40.18），差异有统计学意义（P＜0.05）。结论：推拿手法可能通过改善成肌调节因子Myf-5、Myogenin表达来延缓失神经支配后骨骼肌的萎缩，远期疗效较NGF良好。 |
| 英文摘要: |
| Objective: To observe the action effects and ways of tuina therapy in delaying muscle atrophy after peripheral nerve injury. Method: Male rabbits were randomly divided into sham-operated group, model group, NGF group, tuina group. Then each group were randomly divided into 5 small groups:2 weeks, 3 weeks, 1 month, 2 months, 4 months. The ratio of gastrocnemius weight was decided by contralateral and ipsilateral gastrocnemiuses, mRNA level of Myf-5 and Myogenin were measured by Real-time Polymerase Chain Reaction (qRT-PCR) after 2 weeks, 3 weeks, 1 month, 2 months, 4 months after treatment. Results:①gastrocnemius weight ratio results: compared to model group, the ratio of gastrocnemius weight in NGF group had significantly increase at 2weeks(0.79±0.03)vs(0.53±0.01),3weeks(0.70±0.06)vs(0.41±0.03),1month(0.58±0.04)vs(0.40±0.03)and 2months(0.44±0.05)vs(0.38±0.05)points (P< 0.05);compared to model group, the ratio of gastrocnemius weight in tuina group had significantly increase at 2weeks(0.72±0.05)vs(0.53±0.01),3 weeks(0.55±0.04)vs(0.41±0.03),1month(0.55±0.09) vs(0.40±0.03),2months(0.49±0.03)vs(0.38±0.05) and 4months(0.50±0.03)vs(0.38±0.05)points (P<0.05); compared to NGF group, the ratio of gastrocnemius weight in tuina group was significantly decreased at 2weeks(0.72±0.05)vs(0.79±0.03),3weeks(0.55±0.04)vs(0.70±0.06)(P < 0.05)，the ratio of gastrocnemius weight in tuina group had significantly increase at 4months(0.50±0.03)vs(0.42±0.02) (P<0.05).②mRNA level of Myf-5: compared to model group, the mRNA level of Myf-5 in NGF group had significantly decrease at 2weeks(18.50±1.79)vs(40.91±10.31),3 weeks(12.52±0.58)vs(25.25±1.27),1month(5.30±1.76)vs(13.91±1.40) and 2months(2.82±0.23)vs(8.83±0.86) points (P<0.05); compared to model group, the mRNA level of Myf-5 in tuina group had significantly decrease at 2weeks(20.48±2.20)vs(40.91±10.31),but increased at 1month(16.50±1.17)vs(13.91±1.40),2months(12.20±0.84)vs(8.83±0.86)and 4months (5.32±0.60)vs(3.82±0.54)points (P<0.05); compared to NGF group, the mRNA level of Myf-5 in tuina group was significantly increased at 3 weeks(37.21±9.10)vs (12.52±0.58),1month(16.50±1.17)vs(5.30±1.76),2months(12.20±0.84)vs(2.82±0.23) and 4 months(5.32±0.60)vs(3.88±0.57) points (P<0.05).③mRNA level of Myogenin: compared to model group, the mRNA level of Myogenin in NGF group had significantly decrease at 2weeks(94.31±23.87)vs(185.92±48.71),3weeks(165.95±29.58)vs(359.21±116.88),1month(156.59±64.76)vs(597.50±146.66)and 4months(4.05±0.51)vs (63.13±12.24)points (P<0.05);compared to model group, the mRNA level of Myogenin in tuina group was significantly increased at 2months(1026.75±132.63)vs (168.84±31.85),but decreased at 4months(5.74±1.35)vs(63.13±12.24) points (P<0.05);compared to NGF group, the mRNA level of Myogenin in tuina group had significantly increase at 2weeks(209.80±91.25)vs (94.31±23.87),3weeks(482.37±97.95)vs(165.95±29.58),1month(667.73±97.95)vs(156.59±64.76) and 2months(140.63±40.18)vs(1026.75±132.63) points(P<0.05).Conclusions: Tuina manipulation may delay skeletal muscle atrophy and denaturation, promote activating mRNA level of Myf-5 and Myogenin after peripheral nerve injury, the long-term effect is better than the treatment of NGF. |
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