| 戴如冰,陈 骅.芹菜素对深II度烧伤大鼠急性肺损伤保护作用的研究[J].浙江中西医结合杂志,2021,31(3): |
| 芹菜素对深II度烧伤大鼠急性肺损伤保护作用的研究 |
| The protective effect of Apigenin on acute lung injury in rats with deep second degree burn |
| 投稿时间：2020-09-20 修订日期：2021-01-25 |
| DOI： |
| 中文关键词: 芹菜素 烧伤 肺损伤 氧化应激 炎症 |
| 英文关键词:Apigenin Burn Lung injury Oxidative stress Inflammation |
| 基金项目:浙江省嘉兴市科技计划项目(2019AY53086) |
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| 中文摘要: |
| 目的 研究芹菜素对深II度烧伤大鼠急性肺损伤的保护作用及其机制。方法 将120只实验用SD大鼠随机平均分为假烧伤组(生理盐水)、模型组(生理盐水)、地塞米松5mg/kg组和芹菜素12.5、25、50 mg/kg组。除假手术组外,其余各组大鼠均采用106℃、0.03 MPa压力持续5s的方法制备深II度烧伤大鼠模型。给药治疗14d后,检测各组大鼠肺功能指标,计算肺指数,HE染色法行肺组织病理学检查并行损伤评分,TUNEL法观察肺细胞凋亡,透射电子显微镜观察肺细胞超微结构改变；生化分析法检测肺组织髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和MDA含量,ELISA法检测炎症细胞因子[肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、IL-6]含量。结果 与模型组比较,地塞米松5mg/kg组和芹菜素25、50 mg/kg组大鼠呼吸频率降低[(100.13±5.69)次/min、(108.72±6.81)次/min、(98.27±5.58)次/min比(116.04±7.25)次/min,P<0.05或P<0.01]、肺泡通透指数降低[(2.74±0.42)、(2.88±0.46)、(2.19±0.37)比(3.92±0.57),P<0.01]、氧分压(PaO2)升高[(6.71±1.30) KPa、(6.59±1.34)KPa、(8.24±1.76)KPa比(4.05±0.86)KPa,P<0.01]、肺指数降低[(5.16±0.65)、(5.18±0.71)、(4.53±0.68)比(5.93±0.85),P<0.05或P<0.01]；肺组织形态结构病变、细胞凋亡状况和肺细胞超微结构病变均明显改善,损伤评分降低[(7.76±0.91)分、(7.15±1.12)分、(4.89±0.75)分比(10.73±1.74)分,P<0.01]和凋亡指数(AI)降低[(36.90±6.45)%、(34.19±6.28)%、(22.14±4.67)%比(51.27±8.03)%,P<0.01]；SOD活性升高[(58.47±8.70)U/mg、(62.98±9.04)U/mg、(69.13±9.37)U/mg比(48.19±8.24)U/mg,P<0.05或P<0.01]且MDA含量降低[(4.56±0.92)nmol/mg、(4.29±0.82)nmol/mg、(3.02±0.71) nmol/mg比(5.64±1.10) nmol/mg,P<0.05或P<0.01]、TNF-α含量降低[(84.82±11.03)pg/mL、(94.17±12.68)pg/mL、(63.84±9.01)pg/mL比(126.75±15.41) pg/mL,P<0.01]、IL-1β含量降低[(0.69±0.16)pg/mL、(0.98±0.17)pg/mL、(0.57±0.13)pg/mL比(1.40±0.29)pg/mL,P<0.01]、IL-6含量降低[(104.17±13.99)pg/mL、(135.93±15.02)pg/mL、(97.85±12.16)pg/mL比(183.82±18.15) pg/mL,P<0.01]；芹菜素25、50 mg/kg组MPO活性升高[(1.29±0.37)U/g、(1.40±0.39)U/g比(0.99±0.24)U/g，P<0.05]、CAT活性升高[(24.05±5.36)U/mg、(26.24±5.48)U/mg比(17.92±4.33)U/mg，P<0.05或P<0.01]。与地塞米松5mg/kg组比较，芹菜素50 mg/kg组肺泡通透指数降低[(2.19±0.37)比(2.74±0.42)，P<0.01]、PaO2升高[(8.24±1.76)KPa比(6.71±1.30) KPa，P<0.05]、肺指数降低[(4.53±0.68)比(5.16±0.65)，P<0.05]，肺组织损伤评分降低[(4.89±0.75)分比(7.76±0.91)分，P<0.01]、AI降低[(22.14±4.67)%比(36.90±6.45)%，P<0.01]，MPO活性升高[(1.40±0.39)U/g比(1.07±0.28)U/g，P<0.05]、SOD活性升高[(69.13±9.37)U/mg比(58.47±8.70)U/mg，P<0.05]，MDA含量降低[(3.02±0.71) nmol/mg比(4.56±0.92)nmol/mg，P<0.01]、TNF-α含量降低[(63.84±9.01)pg/mL比(84.82±11.03)pg/mL，P<0.01]。结论 芹菜素对深II度烧伤大鼠急性肺损伤具有保护作用，可能与抑制氧化应激和炎症反应有关。 |
| 英文摘要: |
| Objective To investigate the protective effect of Apigenin on acute lung injury in rats with deep second degree burn and its mechanism. Methods 120 experimental SD rats were randomly divided into sham burn group (normal saline), model group (normal saline), dexamethasone 5mg/kg group and apigenin 12.5, 25, 50 mg/kg group. Except for sham operation group, the rats in other groups were prepared with a deep second degree burn rat model at 106°C, 0.03 MPa pressure for 5 seconds. After the drugs were given for 14d, the lung function indexes of each group of rats were detected, the lung index was calculate, perform the lung histopathological was examined by HE staining method and scored, the lung cell apoptosis was observed by TUNEL method, the ultrastructural changes of lung cells was observed by transmission electron microscope. The myeloperoxidase (MPO), superoxide dismutase (SOD), catalase (CAT) activity and MDA content in lung tissue were detected by biochemical analysis method; the content of inflammatory cytokines [tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6)] were detected by ELISA method. Results Compared with the model group, the respiratory rate [(100.13±5.69)time/min, (108.72±6.81) time/min, (98.27±5.58) time/min vs (116.04±7.25) time/min, P<0.05 or P<0.01]and alveolar permeability index [(2.74±0.42), (2.88±0.46), (2.19±0.37) vs (3.92±0.57), P<0.01] of the rats in Apigenin 25, 50 mg/kg group and dexamethasone 5 mg/kg group were decreased, PaO2 increased [(6.71±1.30) KPa, (6.59±1.34)KPa, (8.24±1.76)KPa vs (4.05±0.86)KPa, P<0.01] and lung index [(5.16±0.65), (5.18±0.71), (4.53±0.68) vs (5.93±0.85), P<0.05 or P<0.01] decreased. The lung tissue morphological/structural lesions, cell apoptosis and lung cell ultrastructural lesions were significantly improved, and the injury score [(7.76±0.91) score, (7.15±1.12) score, (4.89±0.75) score vs (10.73±1.74) score, P<0.01] and apoptosis index (AI) [(36.90±6.45)%, (34.19±6.28)%, (22.14±4.67)% vs (51.27±8.03)%, P<0.01] were reduced. The activity of SOD [(58.47±8.70)U/mg, (62.98±9.04)U/mg, (69.13±9.37)U/mg vs (48.19±8.24)U/mg, P<0.05 or P<0.01] was increased and the contents of MDA[(4.56±0.92)nmol/mg, (4.29±0.82)nmol/mg, (3.02±0.71) nmol/mg vs (5.64±1.10) nmol/mg, P<0.05 or P<0.01], TNF-α [(84.82±11.03)pg/mL, (94.17±12.68)pg/mL, (63.84±9.01)pg/mL vs (126.75±15.41) pg/mL, P<0.01], IL-1β[(0.69±0.16)pg/mL, (0.98±0.17)pg/mL, (0.57±0.13)pg/mL vs (1.40±0.29)pg/mL, P<0.01], IL-6 [(104.17±13.99)pg/mL, (135.93±15.02)pg/mL, (97.85±12.16)pg/mL vs (183.82±18.15) pg/mL, P<0.01] were decreased. And the MPO activity of the Apigenin 25, 50 mg/kg groups increased [(1.29±0.37)U/g, (1.40±0.39)U/g vs (0.99±0.24)U/g, P<0.05]; the CAT activity increased [(24.05±5.36)U/mg, (26.24±5.48)U/mg vs (17.92±4.33)U/mg, P<0.05 or P<0.01]. Compared with dexamethasone 5mg/kg group group, the alveolar permeability index[(2.19±0.37) vs (2.74±0.42), P<0.01] of the rats in Apigenin 50 mg/kg group was decreased, PaO2 increased[(8.24±1.76)KPa vs (6.71±1.30) KPa, P<0.05] and lung index decreased [(4.53±0.68) vs (5.16±0.65), P<0.05]. The injury score [(4.89±0.75) score vs (7.76±0.91) score, P<0.01] and AI [(22.14±4.67)% vs (36.90±6.45)%, P<0.01] were reduced. The activity of MPO [(1.40±0.39)U/g vs (1.07±0.28)U/g, P<0.05], SOD [(69.13±9.37)U/mg vs (58.47±8.70)U/mg, P<0.05] were increased and the contents of MDA [(3.02±0.71) nmol/mg vs (4.56±0.92) nmol/mg, P<0.01], TNF-α [(63.84±9.01)pg/mL vs (84.82±11.03)pg/mL, P<0.01] were decreased. Conclusion Apigenin has protective effect on acute lung injury in rats with deep second-degree burns, which may be related to its effects of inhibition of oxidative stress and inflammation. |
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