| 李春青,过建春,荀运浩,王薇薇,石伟珍.慢加急性肝衰竭合并自发性腹膜炎肠道菌群特征分析[J].浙江中西医结合杂志,2021,31(11): |
| 慢加急性肝衰竭合并自发性腹膜炎肠道菌群特征分析 |
| Characteristics of intestinal flora in patients with acute-on-chronic liver failure complicated with spontaneous bacterial peritonitis |
| 投稿时间：2020-11-15 修订日期：2021-03-26 |
| DOI： |
| 中文关键词: 肝衰竭，慢加急性 自发性细菌性腹膜炎 肠道菌群 16S rDNA高通量测序 |
| 英文关键词:Liver failure,Spontaneous bacterial peritonitis,Intestinal flora,high-throughput 16S rDNA sequencing |
| 基金项目:浙江省中医药科技计划项目(2018 ZB109，2021 ZB234) ；国家“十三五”科技重大专项(No.2018ZX10725506-002、2018ZX10303502-003)；过建春名老中医专家传承工作室（No.GZS2020035） |
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| 中文摘要: |
| 目的：分析慢加急性肝衰竭并发自发性细菌性腹膜炎患者肠道菌群特征，探讨肠道菌群特征与其预后转归的关系。方法：选择37例慢加急性肝衰竭住院患者，提取粪便肠道菌群DNA行高通量测序，对所有入组患者依据临床经验及指南等进行规范化治疗，入院时并发SBP的病例及住院期间发生SBP病例均为B组，其余未并发SBP的病例为A组，应用生物信息分析软件对结果进行统计分析。结果：在属的水平上腹膜炎组患者肠道菌群丰度较高的为拟杆菌属、大肠杆菌志贺菌属、肠球菌属、副拟杆菌属、韦荣氏球菌属、魏斯氏菌属，而未发生腹膜炎组患者肠道菌群丰度较高的菌属主要有拟杆菌属、乳酸菌属、克雷伯菌属、普雷沃菌属、链球菌属、韦荣氏球菌属。Venn图分析结果显示无腹膜炎患者肠道菌群物种数量多于腹膜炎组。PCoA分析结果显示两组样本之间物种丰度有着明显的差异。功能分析结果显示两组间在氨基酸、碳水化合物、无机离子的转运代谢途径存在明显差异（P＜0.05）。结论：慢加急性肝衰竭并发SBP与其肠道菌群特征有着密切联系，肠道菌群对病情预后及转归有着一定的影响。 |
| 英文摘要: |
| Objective Analysis the characteristics of intestinal flora in patients with acute-on-chronic liver failure complicated with spontaneous bacterial peritonitis.
To explore the relationship between intestinal flora characteristics and prognosis. Methods 37 patients with acute-on-chronic liver failure were recruited in this study. The excrements in the patients were collected to test the intestinal flora，and the fecal intestinal flora DNA was obtained by fecal bacterial genome DNA extraction kits. The 16S rDNA of metagenomics sequencing technology was applied to analyze the intestinal flora. All the patients were treated according to clinical experience and guidelines. The patients diagnosed as SBP after admission and those with SBP during hospitalization were in group B, and the others without SBP were in group A. the sequencing results were analyzed by bioinformatics analysis software and statistical software. Results At the level of genus, Bacteroides、Escherichia/Shigella、Enterococcus、Parabacteroides、Veillonella、Weissella were higher in patients with SBP, while Bacteroides、Lactobacillus、Klebsiella、Prevotella、Streptococcus and Veillonella were higher in patients without SBP. Venn plot analysis showed that the number of intestinal flora in patients without peritonitis was more than that in patients with peritonitis. PCoA analysis showed that there were significant differences in species richness between the two groups. The results of functional analysis showed that there were significant differences in the transport and metabolism of amino acids, carbohydrates and inorganic ions between the two groups（P＜0.05）.Conclusion There is a close relationship between SBP and the characteristics of intestinal flora in patients with acute-on-chronic liver failure. Intestinal flora plays an important role in the prognosis of patients. |
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