| 朱文芳,林金辉.五味子乙素逆转乳腺癌细胞多柔比星耐药的机制研究[J].浙江中西医结合杂志,2021,31(11): |
| 五味子乙素逆转乳腺癌细胞多柔比星耐药的机制研究 |
| Study on the Mechanism of Schizandrin B on Reversing Doxorubicin Resistance in Breast Cancer Cells |
| 投稿时间：2021-01-16 修订日期：2021-10-11 |
| DOI： |
| 中文关键词: 五味子乙素 乳腺癌 多柔比星 耐药 P-gp STAT3信号通路 |
| 英文关键词:Schizandrin B breast cancer doxorubicin drug resistance P-gp STAT3 signaling pathway |
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| 中文摘要: |
| 目的 探讨五味子乙素逆转乳腺癌细胞MCF7多柔比星耐药的作用及其机制。方法 采用MTT法检测多柔比星(Doxorubicin, DOX)及DOX联合五味子乙素(Schisandrin B, Sch B)对乳腺癌细胞MCF7及耐药细胞MCF7/ADR的增殖抑制效果,计算IC50以及耐药倍数；将MCF7/ADR建立DOX组、Sch B组及联用组,用集落实验检测细胞集落数情况,用流式凋亡术检测细胞凋亡情况,用细胞划痕实验检测细胞划痕愈合情况； Western blot检测P-gp、MRP1、p-STAT3、STAT3和c-Myc的蛋白相对表达水平。结果 MCF7及耐药细胞MCF7/ARD对DOX的IC50值分别为(4.01 ± 0.15)μmol/L和(50.78 ± 0.67)μmol/L,耐药倍数为12.67；DOX联合SchB后对MCF7及MCF7/AD的IC50值分别为(2.28 ± 0.34)μmol/L和(13.24 ± 0.58)μmol/L,耐药倍数为5.81。相对于DOX组,联用组的细胞集落数明显被抑制,细胞凋亡率增加,细胞划痕愈合率降低(P＜0.05)。同时,联用组下调P-gp、MRP1、p-STAT3以及c-Myc的蛋白表达水平(P＜0.05)。结论 五味子乙素通过抑制P-gp蛋白上调及STAT3信号通路逆转乳腺癌细胞MCF7多柔比星耐药。 |
| 英文摘要: |
| Objective To investigate the reversal effect of Schizandrin B on doxorubicin-induced breast cancer cells MCF7. Methods MTT method was used to detect the proliferation effects of Doxorubicin (DOX) and DOX combined with Schisandrin B (Sch B) on breast cancer cells MCF7 and drug-resistant cells MCF7/ADR, and calculate the IC50 and reversal fold. Establish DOX group, Sch B group and combination group with MCF7/ADR, colony experiment was used to detect the number of cell colonies, use flow cytometry was applied to detect cell apoptosis; cell scratch experiment was used to detect cell scratch healing. The expression levels of P-gp, MRP1, STAT3, STAT3 and c-Myc were analyzed by western Blot. Results The IC50 values ??of MCF7 and MCF7/ARD against DOX were (4.01 ± 0.15) μmol/L and (50.78 ± 0.67) μmol/L, respectively, and the reversal fold was 12.67; The IC50 values ??of MCF7 and MCF7/ARD against DOX combined with SchB were (2.28 ± 0.34) μmol/L and (13.24 ± 0.58) μmol/L, respectively, and the multiple of resistance was 5.81. Compared with the DOX group, the number of cell colonies in the combination group was significantly suppressed, and the apoptosis rate was increased, and cell scratch healing rate was reduced (P＜0.05). At the same time, after treatment of DOX and SchB, the protein expression levels of P-gp, MRP1, p-STAT3 and c-Myc were increased (P＜0.05). Conclusion Schizandrin B can reverse the doxorubicin resistance of breast cancer cells MCF7 by inhibiting the P-gp protein and STAT3 signaling pathway, respectively. |
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