| 王仙伟,雷虹,何欣威,朱峰,柯绍发△.基于调控ABCA1表达探讨升麻苷抗动脉粥硬化的作用机制*[J].浙江中西医结合杂志,2021,31(11): |
| 基于调控ABCA1表达探讨升麻苷抗动脉粥硬化的作用机制* |
| Anti - atherosclerosis Mechanism of Cimicifugoside Based on Regulation of ABCA1 Expression |
| 投稿时间：2021-02-07 修订日期：2021-10-11 |
| DOI： |
| 中文关键词: 动脉粥样硬化 升麻苷 炎症因子 胆固醇外排 |
| 英文关键词:Atherosclerosis Cimicifugoside Inflammatory factors Cholesterol efflux. |
| 基金项目:浙江省医药卫生项目(2017KY705)；台州市科技局项目(1701KY15、20ywb111) |
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| 中文摘要: |
| 目的 研究升麻苷对人单核细胞(THP-1)源性巨噬泡沫细胞炎症因子IL-6, TNF-α的影响,调控ABCA1表达,探讨其抗动脉粥样化的机制。方法 使用适当升麻苷(16、64 μg/ml)作用于诱导成功的THP-1源性巨噬泡沫细胞,ELISA测定细胞上清培养液中IL-6、TNF-α表达,Western印迹测定IL-6、TNF-α以及ABCA1的表达量,闪烁计数法测定细胞内胆固醇流出率。结果 升麻苷浓度≤64ug/ml时,对泡沫细胞无明显毒性；ELISA测定显示不同浓度升麻苷能够降低IL-6、TNF-α的表达(P＜0.01; 0.05)；Western印迹测定显示不同浓度升麻苷能够降低IL-6、TNF-α的表达,增加ABCA1的表达(P＜0.01; 0.05)；闪烁计数法测定结果显示不同浓度升麻苷能够提升细胞胆固醇流出率(P＜0.01; 0.05)。结论 升麻苷可抑制THP-1源性巨噬泡沫细胞炎症因子IL-6、TNF-α表达,提升ABCA1表达,促进胆固醇外排,从而达到抗炎及抑制动脉粥样硬化的作用。 |
| 英文摘要: |
| Objective To explore the effect of Cimicifugoside on the inflammatory factors IL-6 and TNF-α of human monocyte (THP-1)-derived macrophage foam cells, regulate the expression of ABCA1, and explore its anti-atherogenic mechanism. Methods The expression of IL-6, TNF-α and ABCA1 was determined by Western blotting using appropriate Cimicifugoside (16, 64 μg/ml) on THP-1 macrophage foam cells; Determination of IL-6 and TNF-α in cell supernatant by ELISA; determination of intracellular cholesterol efflux by scintillation counting. Results The results of CCK8 showed that there was no obvious toxicity to foam cells when the concentration of Cimicifugoside was ≤64ug/ml. Cimicifugoside could reduce the expression of IL-6、TNF-α (P<0.01; 0.05), increase the expression of ABCA1(P<0.01; 0.05) and the cholesterol efflux rate (P<0.05). Conclusion Cimicifugoside can inhibit the expression of inflammatory factors IL-6 and TNF-α in THP-1-derived macrophage foam cells, increase the expression of ABCA1, and promote cholesterol efflux, thereby achieving anti-inflammatory and inhibiting atherosclerosis effects. |
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