| 李慧,陈李兰,李蕊,戴园园.人参皂苷Rg1对发育期癫痫大鼠认知功能的影响[J].浙江中西医结合杂志,2022,32(4): |
| 人参皂苷Rg1对发育期癫痫大鼠认知功能的影响 |
| The Influence of Ginsenoside Rg1 on The Cognitive Dysfunction by Epilepsy in Pubertal Rat |
| 投稿时间：2021-08-26 修订日期：2022-03-16 |
| DOI： |
| 中文关键词: 癫痫 认知障碍 miR-124 人参皂苷Rg1 |
| 英文关键词:epilepsy Cognitive impairment miR-124 Ginsenoside Rg1 |
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| 中文摘要: |
| [摘要] 目的 研究人参皂苷Rg1对发育期癫痫大鼠的认知功能的影响。方法 14d SD大鼠腹腔注射红藻氨酸构建慢性癫痫模型；所有实验大鼠按随机数字表法分为生理盐水对照组、癫痫模型组、人参皂苷Rg1低剂量（10mg/kg）、中剂量（30mg/kg）及高剂量组(60mg/kg)；每组各10只；Morris水迷宫测定大鼠的学习记忆功能；RT-qPCR法检测NRSF mRNA及miR-124的表达，Westernblot法检测NRSF蛋白的表达，尼氏染色(Nissl染色)检测海马结构的病理改变。结果 与对生理盐水组比较，红藻氨酸组大鼠逃避潜伏期延长（9.02±6.34 比39.05±8.74），空间探索缩短（44.23±8.12比13.23±5.43），海马内NRSF（0.31±0.04比0.71±0.11）及 miR-124（0.41±0.07比0.60±0.08）明显增加，差异有统计学意义（P<0.05），与红藻氨酸组比较，Rg1高剂量组大鼠逃避潜伏期缩短（39.05±8.74比17.09±7.56），空间探索延长（13.23±5.43比35.27±5.49），海马内NRSF（0.71±0.11比0.50±0.06）表达减少，而miR-124（0.60±0.08比0.84±0.07）的表达量增加，差异有统计学意义（P<0.05）。Nissl染色结果显示生理盐水组大鼠海马CA3区锥体细胞排列整齐、紧密，细胞结构完整，神经元内布满深蓝色颗粒状的尼氏小体；与生理盐水组相比，红藻氨酸组大鼠海马CA3区锥体细胞排列疏松，部分神经元胞体皱缩、碎裂，核固缩，胞质内尼氏小体大量脱失；不同Rg1治疗组大鼠海马CA3区神经元皱缩、碎裂、尼氏小体减少、脱失较红藻氨酸组减轻，以人参皂苷Rg1高剂量组减轻明显。结论 人参皂苷Rg1可以改善癫痫所致的认知功能障碍，这可能与减少NRSF的表达量及增加miR-124的表达量有关，且成剂量依赖性。 |
| 英文摘要: |
| 【Abstract】Objective To observe the effect of ginsenoside Rg1 on cognitive function in pubertal rats with chronic epilepsy. Methods 14-day-old SD rats to build chronic epilepsy model was established through intraperitoneal injection of KA. All experimental rats were randomly divided into control group(NS group)，Epilepsy model group (KA group)，ginsenoside Rg1 (set 10 mg/kg, 30 mg/kg, 60 mg/kg three doses) intervention group，each group of 10 rats．The learning and memory function of pubertal SD rats were analysed by Morris water maze. The levels of NRSF mRNA and miR-124 in hippocampus tissue were analysed by real time fluorescence quantitative PCR(RT-qPCR). The levels of NRSF protein in hippocampus tissue were analysed by western blot. Results Compared with the NS group , KA group, the location navigation test escape latency period（9.02±6.34 vs 39.05±8.74） was delayed and the space exploration（44.23±8.12 vs 13.23±5.43） was shortened. NRSF（0.71±0.11 vs 0.50±0.06）,MiR-124（0.60±0.08 vs 0.84±0.07）increased significantly in the hippocampus(P<0.05). Compared with KA group , Rg1-60mg escape latency period（39.05±8.74 vs 17.09±7.56） was shortened and the space exploration （13.23±5.43 vs 35.27±5.49）was delayed. The levels of NRSF（0.71±0.11 vs 0.50±0.06） was decreased but miR-124（0.60±0.08 vs 0.84±0.07） increased in hippocampus(P<0.05). Nissl staining showed that rats in NS group had neat and tight pyramidal cells, complete cell structure, neurons covered with dark blue particles, loose, some neurons wrinkled, fragmented, and nuclear consolidation, and large loss in KA group in different Rg1 treatment groups in ginsenoside Rg1-60mg group. Conclusion ginsenoside Rg1 can improve the cognitive dysfunction induced by epilepsy, which maybe related to reducing the amount of NRSF expression and increasing the amount of miR-124 expression. |
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