| 刘丽琴.解毒软肝合剂质量控制方法研究 [J].浙江中西医结合杂志,2022,32(3): |
| 解毒软肝合剂质量控制方法研究 |
| Study on Quality Control Method of Jieduruangan Mixture |
| 投稿时间：2021-08-26 修订日期：2021-12-22 |
| DOI： |
| 中文关键词: 解毒软肝合剂 炙甘草 叶下珠 赤芍 芍药苷 质量控制 |
| 英文关键词:Jieduruangan mixture Glycyrrhizae Radix et Rhizoma Praeparata Cummelle Phyllanthus urinaria L Paeoniaeradix Rubra paeoniflorin quality control |
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| 中文摘要: |
| 目的 建立解毒软肝合剂的质量评价体系。方法 采用TLC法对合剂中炙甘草、叶下珠、赤芍进行定性鉴别；使用HPLC法定量检测合剂中芍药苷含量；选择Diamonsil C18色谱柱(250×4.6 mm,5 μm)；选择乙腈(A)-0.1%磷酸水溶液(B)作为流动相,进行梯度洗脱,条件为0~30 min 14% A; 30~60 min 14~90% A; 60~61 min 90~14% A;61~70 min 14% A；以230 nm作为检测波长；以1.0 mL.min-1为流动性流速；柱温设定为30 ℃；每次进样10μL。结果 TLC方法展开的色谱图分离度好,斑点清晰。HPLC方法结果显示,芍药苷在0.1177～0.5885μg范围内线性关系良好,平均回收率达到99.06%。结论 TLC与HPLC相结合的质量评价体系呈现较好的精密度、重复性、稳定性,可运用于解毒软肝合剂的质量控制。
关键词：解毒软肝合剂；炙甘草；叶下珠；赤芍；芍药苷；质量控制 |
| 英文摘要: |
| Study on Quality Control Method of Jieduruangan Mixture
LIIU Liqin1*, WANG Ying1,CHEN Hongbin2(1.Zhejiang Integrated Traditional and Western Medicine Hospital, Hangzhou 310003, China;2.Zhejiang Huisong Pharmaceutical Co., Ltd., Hangzhou 310005, China)
Objective: abstract To establish the quality control method of Jieduruangan mixture. Methods Thin layer chromatography(TLC)was used to identify Glycyrrhizae Radix et Rhizoma Praeparata Cum melle;Phyllanthus urinaria L;Paeoniaeradix Rubr. Establish an HPLC method for determination the content of paeoniflorin. The method was performed with Diamonsil C18(4.6 mm×250 mm,5?μm) column and gradient elution with acetonitrile (A)-0.1% phosphoric acid (B) as the mobile phase,gradient elution;0～30 min 14% A; 30~60min 14~90 A; 60~61 min 90~14 A;61~70min 14% A, the detection wavelength was 230 nm and the flow rate was 1.0 mL·min-1, the column temperature was 30℃ and the injection volume was 10μL. Results TLC showed clear spots and good resolution. Paeoniflorin showed a good linear relationship in the range of 0.1177～0.5885μg, the average recovery of paeoniflorin was 99.06%. Conclusion The method has good precision, repeatability, stability,it can be used for the quality control of Jieduruangan mixture.
KEY WORDS: Jieduruangan mixture; Glycyrrhizae Radix et Rhizoma Praeparata Cum melle; Phyllanthus urinaria L;Paeoniaeradix Rubra; Paeoniflorin; Quality control |
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