| 尹剑.NEC-1在大鼠ICH后继发性脑损伤中的作用研究[J].浙江中西医结合杂志,2022,32(3): |
| NEC-1在大鼠ICH后继发性脑损伤中的作用研究 |
| Therapeutic effect and mechanism of NEC-1 on secondary brain injury after spontaneous intracerebral hemorrhage in rats |
| 投稿时间：2021-09-04 修订日期：2021-12-15 |
| DOI： |
| 中文关键词: NEC-1 脑出血 脑水肿 RIP1/RIP3 |
| 英文关键词:NEC-1 intracerebral hemorrhage Cerebral edema RIP1/RIP3 |
| 基金项目:杭州市卫生健康委员会资助项目(2018A40) |
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| 中文摘要: |
| 目的 探讨NEC-1对大鼠自发性脑出血后继发性脑损伤的治疗作用及机制。方法 成年SD大鼠随机分为假手术组，脑出血+生理盐水组和脑出血+NEC-1组。采用测大脑干湿重法检测大鼠脑组织含水量，应用Western blotting法检测脑组织中RIP1/RIP3，TNF-α， IL-1β蛋白水平的表达情况，采用TUNEL法检测脑组织神经细胞凋亡情况。结果 与生理盐水组相比，NEC-1能够明显减轻大鼠脑组织含水量，下调RIP1/RIP3, TNF-α, IL-1β蛋白的表达，减少神经细胞凋亡，组间差异比较有统计学意义（P<0.05）。结论 NEC-1能够减轻ICH继发性脑损伤后脑水肿，具有显著的神经保护作用，可能是通过调节RIP1/RIP3的作用，为继发性脑损伤的治疗提供了新的思路。 |
| 英文摘要: |
| Objective To investigate the therapeutic effect and mechanism of Necrostain-1 on secondary brain injury after spontaneous intracerebral hemorrhage in rats. Methods Adult SD rats were randomly divided into sham operation group, intracerebral hemorrhage + normal saline group and intracerebral hemorrhage + NEC-1 group. The brain dry and wet weight method was used to detect the water content of the rat brain tissue, Western blotting were used to detect the expression of RIP1/RIP3, TNF-α, IL-1β protein levels in the brain tissue, and the TUNEL method was used to detect the apoptosis of neurons in brain tissue. Results Compared with the normal saline group, NEC-1 can significantly reduce the brain water content of rat, down-regulate the expression of RIP1/RIP3, TNF-α, IL-1β protein, and reduce nenronal apoptosis. The difference between the groups is statistically significant. (P<0.05). Conclusion NEC-1 can reduce brain edema after ICH secondary brain injury and has a significant neuroprotective effect. It may provide a new idea for the treatment of ICH secondary brain injury by regulating RIP1/RIP3. |
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