| 姚成.益气祛痰解毒方通过调控FRMD6 抑制非小细胞肺癌进展的作用机制研究[J].浙江中西医结合杂志,2022,32(9): |
| 益气祛痰解毒方通过调控FRMD6 抑制非小细胞肺癌进展的作用机制研究 |
| Mechanism of Yiqi Qutan Jiedu Decoction in Inhibiting the Progression of Non-small Cell Lung Cancer by FRMD6 |
| 投稿时间：2021-10-22 修订日期：2022-08-26 |
| DOI： |
| 中文关键词: 非小细胞肺癌 益气祛痰解毒方 PC9细胞 FRMD6 c-MET/P13K/Akt通路 |
| 英文关键词:Non-small cell lung cancer Yiqi Qutan Jiedu prescription PC9 cells FRMD c-MET/P13K/Akt pathway |
| 基金项目: |
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| 中文摘要: |
| 探究益气祛痰解毒方对非小细胞肺癌（non-small cell lung cancers，NSCLC）细胞的抑制作用和相关分子机制。方法 构建FRMD6（FERM domain-containing protein 6, FRMD6）敲低PC9细胞株。将PC9细胞分为对照组，shNC组，shFRMD6组，益气祛痰解毒方+shNC组，益气祛痰解毒方+shFRMD6组。采用实时定量PCR（qRT-PCR）检测各组PC9细胞的FRMD6表达水平变化；应用CCK-8法检测各组PC9细胞增殖率；采用Annexin V-FITC/PI法检测试各组PC9细胞凋亡情况；利用Transwell实验检测各组PC9细胞侵袭能力；利用Western blot检测各组FRMD6, c-MET, p-PI3K, PI3K, p-Akt和Akt蛋白表达的变化。结果 CCK8检测结果表明，与对照组相比，益气祛痰解毒方对PC9细胞增殖具有显著抑制效果（P < 0.05）；qRT-PCR和Western blot结果显示，发现益气祛痰解毒方可以上调FRMD6表达。进一步进行qRT-PCR和Western blot结果表明FRMD6敲低PC9细胞株构建成功；益气祛痰解毒方处理能够抑制PC9细胞增殖、侵袭，并促进细胞凋亡；同时，与shNC组相比，益气祛痰解毒方+shNC组均可显著抑制PC9细胞增殖、侵袭并促进细胞凋亡（P < 0.05）。Western blot结果发现，益气祛痰解毒方+shNC组相较于shNC组的c-MET、p-PI3K和p-Akt表达明显降低，说明使用益气祛痰解毒方处理可抑制c-MET/PI3K/AKT通路；此外，与shFRMD6组相比，益气祛痰解毒方+shFRMD6组c-MET、p-PI3K和p-Akt表达显著下调，敲低FRMD6表达会显著减弱益气祛痰解毒方对c-MET/PI3K/AKT通路的抑制作用。结论 益气祛痰解毒方能够抑制肺癌PC9细胞增殖、降低侵袭能力和促进细胞凋亡，其分子机制可能通过FRMD6调控c-MET/PI3K/AKT通路来实现。 |
| 英文摘要: |
| To explore the inhibitory effect and molecular mechanism of Yiqi Qutan Jiedu prescription on non-small cell lung cancer (NSCLC) cells. Methods To construct FRMD6 knockdown PC9 cells. PC9 cells were divided into control group, shNC group, shFRMD6 group, Yiqi Qutan Jiedu Decoction + shNC group, Yiqi Qutan Jiedu Decoction + shFRMD6 group. The expression level of FRMD6 in PC9 cells in each group was detected by qRT-PCR. CCK-8 method was used to detect the proliferation rate of PC9 cells in each group. The apoptosis of PC9 cells in each group was detected by Annexin V-FITC/PI method. Transwell assay was used to detect the invasion ability of PC9 cells in each group. The expressions of FRMD6, c-MET, p-PI3K, PI3K, p-Akt and Akt proteins in each group were checked by Western blot. Results CCK8 indicated compared with control group, Yiqi Qutan Jiedu Decoction had a significant inhibitory effect on the proliferation of PC9 cells (P < 0.05). The results of qRT-PCR and Western blot showed that Yiqi Qutan Jiedu Decoction could up-regulate the expression of FRMD6. The results of qRT-PCR and Western blot showed that FRMD6 knockdown PC9 cells were successfully constructed. Yiqi Qutan Jiedu Decoction could inhibit the proliferation and invasion of PC9 cells and promote apoptosis. At the same time, compared with the shNC group, the Yiqi Qutan Jiedu Decoction + shNC group could significantly inhibit the proliferation, invasion and promote apoptosis of PC9 cells (P < 0.05). Western blot results showed that the expression of c-MET, p-PI3K and p-Akt in Yiqi Qutan Jiedu Decoction + shNC group was significantly lower than that in shNC group, indicating that Yiqi Qutan Jiedu Decoction can inhibit c-MET / PI3K / AKT pathway. Compared with shFRMD6 group, the expressions of c-MET, p-PI3K and p-Akt in Yiqi Qutan Jiedu Decoction + shFRMD6 group were significantly decreased. In addition, knockdown of FRMD6 expression will affect the inhibitory effect of Yiqi Qutan Jiedu Decoction on c-MET/PI3K/AKT pathway. Conclusion Yiqi Qutan Jiedu Decoction can inhibit proliferation, reduce invasion and promote apoptosis of lung cancer PC9 cells, and its molecular mechanism may be achieved by regulating c-MET / PI3K / AKT pathway by FRMD6. |
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