| 夏亮,谢齐贵,陈湛蕾.芍药苷通过抑制Notch-1信号通路影响肝癌细胞增殖、侵袭和凋亡[J].浙江中西医结合杂志,2022,32(6): |
| 芍药苷通过抑制Notch-1信号通路影响肝癌细胞增殖、侵袭和凋亡 |
| Effects of paeoniflorin on the proliferation, invasion and apoptosis of liver cancer cells via inhibiting Notch-1 signaling pathway |
| 投稿时间：2021-10-24 修订日期：2022-04-28 |
| DOI： |
| 中文关键词: 芍药苷 肝癌 增殖 侵袭 凋亡 Notch-1信号通路 |
| 英文关键词:paeoniflorin liver cancer proliferation invasion apoptosis Notch-1 signaling pathway |
| 基金项目: |
|
| 摘要点击次数: 654 |
| 全文下载次数: 16 |
| 中文摘要: |
| 摘要 目的 探讨芍药苷对肝癌细胞增殖、侵袭和凋亡的影响及其机制。方法 使用不同浓度芍药苷（0、25、50、75、100、150 μmol/L）处理人肝癌细胞系HepG2，采用CCK-8 法检测细胞增殖活性。根据芍药苷浓度将HepG2细胞株分为四组：空白对照组（0 μmol/L）、低剂量组（25 μmol/ L）、中剂量组（50 μmol/L）和高剂量组（75 μmol/L）。采用流式细胞仪检测细胞凋亡情况，Transwell 法细胞侵袭迁移能力，Western blot法测定细胞表达Notch-1和Hes-1的水平变化情况。结果 使用不同浓度（0、25、50、75、100、150 μmol/L）芍药苷处理HepG2 细胞24 h后，细胞增殖活性呈剂量依赖性降低[（100.00）％、（83.34±8.06）%、（74.46±7.34）%、（63.59±2.51）%、（55.93±4.05）%、（39.99±6.06）%，P＜0.05]。当根据不同芍药苷处理浓度（0、25、50、75 μmol/L）处理HepG2 细胞24 h后，细胞穿膜数逐渐降低[（85.33±5.51）；（64.33±5.13）；（49.67±3.51）；（30.00±2.00），P＜0.05]；细胞凋亡率逐渐升高[（2.43±0.93）%；（13.37±2.57）%；（22.64±2.19）%；（28.53±1.37）%，P＜0.05]；Notch-1蛋白表达水平逐渐降低[（1.13±0.15）；（0.75±0.05）；（0.55±0.06）；（0.39±0.04），P＜0.05]；Hes-1蛋白表达水平也逐渐降低[（0.80±0.07）；（0.55±0.07）；（0.38±0.04）；（0.17±0.03），P＜0.05]。结论 芍药苷呈剂量依赖性抑制肝癌细胞HepG2增殖、侵袭，并可促进细胞发生凋亡，其机制可能与Notch-1 信号通路相关。 |
| 英文摘要: |
| Abstract Objective To investigate the effects of paeoniflorin on proliferation, invasion and apoptosis of liver cancer cells and its mechanism. Methods Various concentrations of paeoniflorin (0, 25, 50, 75, 100, 150 μmol/L) were treated with the human liver cancer cell line HepG2, and the cell proliferation rate was detected by CCK-8 assay. Then, HepG2 cells were divided into 4 groups according to the concentrations of paeoniflorin: Blank control group (0 μmol/L), low dose group (25 μmol/L), medium dose group (50 μmol/L) and high dose group (75 μmol/L). Flow cytometry assay and transwell assay was used to detected cells apoptosis and invasion ability. Western blot assay was performed to measure the expression levels of Notch-1 and Hes-1 protein. Results Use different concentrations (0, 25, 50, 75, 100, 150 μmol/L) of paeoniflorin to treat HepG2 cells for 24 h, the proliferation rate of cells decreased in a dose-dependent manner [(100.00)%, (83.34±8.06)%, (74.46±7.34)%, (63.59±2.51)%, (55.93±4.05)%, (39.99 ± 6.06)%, P<0.05]. According to different paeoniflorin treatment concentrations (0, 25, 50, 75 μmol/L) for 24 h, the number of invasive cells redued [(85.33±5.51); (64.33±5.13); (49.67±3.51); (30.00 ± 2.00), P<0.05]; the apoptosis rate increased [(2.43±0.93)%; (13.37±2.57)%; (22.64±2.19)%; (28.53±1.37)%, P<0.05]; the expression level of Notch-1 protein decreased [(1.13±0.15); (0.75±0.05); (0.55±0.06); (0.39±0.04), P<0.05]; the expression level of Hes-1 protein also downregulated [(0.80±0.07); (0.55±0.07); (0.38±0.04); (0.17±0.03), P<0.05]. Conclusion Paeoniflorin suppressed the proliferation and invasion, and promoted apoptosis of liver cancer cell line HepG2 in a dose-dependent manner, its mechanism may be related to Notch-1 signaling pathway. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
