| 晏露宁,汝触会,陈爱凤,陈晔,韩佳颖,何飞.基于PKA依赖cAMP信号介导肺胃SP表达探讨中医肺胃相关理论的机制研究[J].浙江中西医结合杂志,2022,32(11): |
| 基于PKA依赖cAMP信号介导肺胃SP表达探讨中医肺胃相关理论的机制研究 |
| Explore the mechanism of relevant theories of TCM lung and stomach based on PKA-dependent cAMP signal-mediated SP expression in lung and stomach |
| 投稿时间：2022-01-14 修订日期：2022-10-19 |
| DOI： |
| 中文关键词: P物质 蛋白激酶A 环磷酸腺苷(cAMP) 肺胃相关 |
| 英文关键词:Substance P Protein kinase A cAMP Lung and stomach related theoriest. |
| 基金项目:浙江省中医药科技计划（2019ZA088）基于PKA依赖cAMP信号介导肺胃SP表达探讨中医肺胃同治法治疗胃食管反流性咳嗽作用机制研究 |
|
| 摘要点击次数: 463 |
| 全文下载次数: 3 |
| 中文摘要: |
| 目的: 观察胃食管反流模型大鼠肺、胃、食管组织中PKA、cAMP、SP的表达量,探讨中医肺胃相关理论的分子机制。方法: 将12只雄性 SD 大鼠,按随机数字表法分正常对照组( n= 6) 、模型组( n= 6) ,模型组用盐酸氯胺酮注射液腹腔注射麻醉大鼠,经口插型号为5F胃管至食管的中、下段,模型组以8滴/min的速率缓慢灌注0.1mmol/L盐酸(含0.5％胃蛋白酶)溶液,每次20分钟,每日1次,连续14日。对照组采用PBS液代替盐酸灌注食管,方法同模型组。提取食管、胃和肺组织标本进行病理学观察,用免疫组化法测定PKA、cAMP、SP表达,并将结果进行线性回归分析。结果: 两组相比,模型组大鼠肺、食管与胃组织中PKA、cAMP与SP的蛋白表达量均极显著升高(P<0.01)。线性回归结果表明:胃与肺组织的PKA和P物质平均光密度呈线性相关.结论: 1.P物质或许是中医肺胃相关的理论基础之一；2. 激活后的PKA／cAMP是促进肺胃组织释放P物质的重要通路,可能介导了胃食管反流咳嗽的病理生理过程,是肺胃相关理论可能的分子机制之一。 |
| 英文摘要: |
| Objective To observe the expression of PKA, cAMP and SP in the lung, stomach, and esophagus tissues of gastroesophageal reflux model rats, and explore molecular mechanisms related to the lung and stomach of traditional Chinese medicine. Methods Twelve male SD rats were divided into normal control group (n=6) and model group (n=6) according to the random number table. The model group was intraperitoneally injected with ketamine hydrochloride injection to lightly anesthetize the rats, and inserted 5F through the mouth The stomach tube was inserted into the middle and lower part of the esophagus, and the model group was slowly perfused with 0.1 mmol/L hydrochloric acid (containing 0.5% pepsin) solution at a rate of 8 drops/min, 20 minutes each time, once a day, for 14 days. In the control group, PBS was used instead of hydrochloric acid to perfuse the esophagus in the same way as the model group. The esophagus, stomach and lung tissue specimens were extracted for pathological observation, and the expression of PKA, cAMP, SP was measured by immunohistochemistry, and the results were analyzed for linear regression analysis. Results Compared with the normal control group, the protein expressions of PKA, cAMP and SP in the lung, esophagus, and stomach tissues of the model group increased significantly (P<0.01). The linear regression analysis results show that the average optical density of PKA and substance P in the stomach and lung tissues is linear correlation. Conclusions 1. Substance P may be one of the theoretical foundations related to the lung and stomach; 2. The activated PKA/cAMP is an important pathway that promotes the release of substance P from the lung and stomach tissue, and may mediate the pathophysiological process of gastroesophageal reflux cough. This may be one of the molecular mechanisms that can explain Lung and stomach related theoriest. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
