| 刘艳丽,周妍妍.五味子醇甲通过Aβ25-35诱导PC12细胞损伤对氧化应激及炎症因子的影响[J].浙江中西医结合杂志,2023,33(1): |
| 五味子醇甲通过Aβ25-35诱导PC12细胞损伤对氧化应激及炎症因子的影响 |
| Effects of Schisandrin A on oxidative stress and inflammatory factors in PC12 cells induced by Aβ25-35 |
| 投稿时间：2022-03-28 修订日期：2022-12-06 |
| DOI： |
| 中文关键词: 阿尔茨海默病 五味子醇甲 PC12细胞 氧化应激 炎性因子 |
| 英文关键词:Alzheimer’s disease Schisandrin A PC12cells oxidativeSstress SinflammatorySfactor |
| 基金项目:国家自然科学基金委面上(81774197)台州市科技计划(20ywb78) |
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| 中文摘要: |
| 目的 探讨五味子醇甲(Schisandrin A,SCH A)通过Aβ25-35诱导PC12细胞损伤对氧化应激及炎症因子的影响。方法 CCK8、流式细胞仪联合检测,设立20 μmol/L Aβ25-35诱导PC12建立细胞损伤模型。MTT、流式细胞仪确立10μg/mL SCH A为本次实验药物剂量。实验分为空白组、模型组、SCH A组。试剂盒检测超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH)表达水平；Western Blot检测白介素-1β(IL-1β)、白介素-6(IL-6)、肿瘤坏死因子(TNF-α)蛋白水平。结果 与模型组比较,SCH A组能提高SOD、GSH水平,降低MDA水平,有显著差异(P<0.01)；能降低IL-1β、IL-6、TNF-α蛋白表达水平,有显著差异(P<0.01)。结论SCH A可能通过降低Aβ25-35诱导PC12细胞的炎性反应,减轻氧化应激,从而发挥细胞保护作用。 |
| 英文摘要: |
| Objective To investigate the effects of (Schisandrin A,SCH A)Son oxidative stress and inflammatory factors of PC12 cells induced by Aβ25-35. Methods CCK8 was combined with flow cytometry to induce PC12 with 20 μmol/L Aβ25-35 to establish cell damage model. MTT and flow cytometry determined 10μg/mL SCH A as the dose of this experiment. The experiment was divided into blank group, model group and SCH A group. The expression levels of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione (GSH) were detected by the kit. Western Blot analysis of interleukin-1 β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor (TNF-α) protein levels. Results SCH A group could increase the level of SOD and GSH and decrease the level of MDA, with significant difference (P<0.01). SCH A could decrease the expression levels of IL-1β, IL-6 and TNF-α with significant difference (P<0.01). Conclusion SCH A may play A protective role in PC12 cells by reducing the inflammatory response induced by Aβ25-35 and alleviating oxidative stress. |
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