| 顾建荣,韩丽萍,吴瑶,赖威旨,何志刚,叶合,楼招欢.乌药对酒精联合高脂饮食诱导的脂肪肝的防治作用研究[J].浙江中西医结合杂志,2023,33(5): |
| 乌药对酒精联合高脂饮食诱导的脂肪肝的防治作用研究 |
| The protective effect of Linderae radix on fatty liver induced by alcohol and high-fat dietJianrong Gu1,2,Yao Wu1,Liping Han1,Weizhi Lai1,Zhigang 3,He 4,Zhaohuan Lou1* |
| 投稿时间：2022-07-01 修订日期：2023-03-27 |
| DOI： |
| 中文关键词: 乌药 酒精性脂肪肝 低密度脂蛋白受体 胆固醇调节元件结合蛋白－1c 内质网胰岛素诱导基因1 |
| 英文关键词:LinderaSRadix alcoholic fatty liver LDLR SREBP-1 Insig1 |
| 基金项目:] 浙江省基础公益研究计划项目(LYY21H280001)；杭州市富阳区科技计划项目(NO. 20191231Y201)；浙江省医学会临床科研(2021ZYC-A101)；浙江省药学会医院药学专项科研资助项目(2021ZYY38)作者：顾建荣,硕士研究生；E-mail：931990143@qq.com*通信作者：楼招欢,医学博士,副研究员；E-mail：zhaohuanlou@zcmu.edu.cn * |
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| 中文摘要: |
| 目的：观察乌药醇提取物(LREE)对酒精性脂肪肝(AFL)保护作用,探讨其可能的作用机制。方法：采用高脂饲料喂养加灌胃不同酒精浓度白酒连续8周建立AFL小鼠模型,造模同时予以LREE干预；于第2、4、6、8周,采血,全自动生化仪检测血清ALT、AST活性及TC、TG水平；实验期末,解剖,取肝脏油红O染色检测肝组织脂肪变程度,RT- PCR检测肝组织LDLR、PPARα、Insig1、SCAP、SREBP-1mRNA表达。结果：LREE能抑制酒精+高脂饲料有诱导的体重增加,改善AFL小鼠异常的脂质水平,降低血清ALT、AST活性,改善肝组织脂质沉积；RT-PCR结果显示,LREE能上调肝组织LDLR、PPARαmRNA表达水平,下调Insig1、SCAP、SREBP-1mRNA表达水平。结论：乌药对高脂联合酒精诱导的酒精性脂肪肝具有良好的保护作用,其机制可能与抑制Insig1-Scap-SERBP1通路活化有关。 |
| 英文摘要: |
| Objective: To investigate the protective effect of ethanol extract of Linderae radix (LREE) on fatty liver disease induced by alcohol and high-fat diet and to further explore the potential mechanism. Methods: Alcoholic fatty liver (AFL) model was established through treatment of mice with high fat diet and liquor with different alcohol concentration for 8 weeks, and LREE was administrated at the same time. Blood samples were obtained at 2, 4, 6, and 8 weeks. The activities of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), cholesterol (TC), and triglyceride (TG) were measured using an automated biochemical analyzer. Liver tissues were harvested and hepatic steatosis in the mice was assessed based on Oil Red O staining. Real time-polymerase chain reaction (RT-PCR) was applied to determine mRNA of LDLR, PPARα, Insig1, SCAP, and SREBP-1 in liver tissues respectively. Results: LREE was demonstrated to suppress weigh gain induced by alcohol and high-fat diet and ameliorated dyslipidemia. LREE also down-regulated the activities of serum ALT and AST, as well as ameliorate hepatic lipid deposition. RT-PCR results showed that LREE could significantly increase the mRNA expressions of the LDLR and PPARα, but decrease the expression of Insig1, SCAP and SREBP-1. Conclusion: The present study indicated that LREE has an attractive protective effect against alcohol- and high-fat diet- induced fatty liver. This function may be related to activation suppressing of Insig1-Scap-SERBP1 signal pathway. |
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