| 刘峰,项艳.黄芩素脂质聚合物纳米粒的构建及对脑缺血性眩晕大鼠作用的研究[J].浙江中西医结合杂志,2023,33(1): |
| 黄芩素脂质聚合物纳米粒的构建及对脑缺血性眩晕大鼠作用的研究 |
| Construction of baicalein lipid polymer nanoparticles and its effect on cerebral ischemic vertigo in rats |
| 投稿时间：2022-07-06 修订日期：2022-12-09 |
| DOI： |
| 中文关键词: 黄芩素 脂质聚合物纳米粒 缺血性眩晕 氧化应激 前庭核 脑源性神经营养因子 |
| 英文关键词:baicalein lipid polymer nanoparticles ischemic vertigo oxidative stress vestibular nucleus brainderived neurotropic factor |
| 基金项目:丽水市科技计划项目(2020SJZC068) |
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| 中文摘要: |
| 目的 构建TAT修饰的载黄芩素（baicalein，Bai）脂质聚合物纳米粒（TAT modified baicalein-loaded lipid polymer nanoparticles，Bai-TAT-LPNs），观察其对脑缺血性眩晕大鼠的作用及作用机制。方法 纳米沉淀法制备Bai-TAT-LPN，透射电镜和纳米颗粒分析仪分别记录其表面形貌和粒径。构建脑缺血性眩晕大鼠模型，设立假手术组、模型组、Bai组、Bai-TAT-LPNs组。跳台实验检测大鼠跳台潜伏期（step down latency，SDL）；血生化仪检测乳酸（lactic acid，LAC）、乳酸脱氢酶（lactic dehydrogenase，LDH）、丙二醛（malondialdehyde，MDA）和超氧化物歧化酶（superoxide dismutase，SOD）水平；免疫组织化学染色法检测脑组织脑源性神经营养因子（brain-derived neurotrophic factor，BDNF）蛋白表达。结果 Bai-TAT-LPNs为粒径为127.0±2.6 nm的球形。相较于假手术组，模型组大鼠表现出较强的SDL，显著下降的前庭神经核组织血流量，以及异常水平的海马组织LAC、MDA、LDH及SOD含量（P＜0.05或P＜0.01）。而与模型组相比，Bai-TAT-LPNs组鼠SDL显著缩短（P＜0.01），前庭神经核组织血流量明显增加（P＜0.01），海马组织LAC、MDA和LDH含量降低，SOD含量增加（P＜0.05或P＜0.01），且效果优于Bai组。Bai组和Bai-TAT-LPNs组BDNF蛋白表达较假手术组明显升高，Bai-TAT-LPNs组升高更为明显（P＜0.01）。结论Bai-TAT-LPNs对脑缺血性眩晕大鼠具有较好的预防作用，该作用可能与增加前庭核血流量、降低氧化损伤及上调脑海马组织BDNF蛋白表达有关。 |
| 英文摘要: |
| Objective Construct TAT-modified baicalein (Bai) lipid polymer nanoparticles (Bai-TAT-LPNs) and observe its effect on cerebral ischemic vertigo. Methods Bai-TAT-LPN was prepared by nanoprecipitation method, and its surface morphology and particle size were recorded by transmission electron microscope and nanoparticle analyzer, respectively. A rat model of cerebral ischemic vertigo was constructed, and sham operation group, model group, Bai group, and Bai-TAT-LPNs group were established. Platform jumping test to detect platform jumping latency (SDL) in rats. Blood biochemical analyzer detected lactic acid (LAC), lactate dehydrogenase (LDH), malondialdehyde (MDA) and superoxide dismutase (SOD) levels. The protein expression of brain-derived neurotrophic factor (BDNF) in brain tissue was detected by immunohistochemical staining. Results Bai-TAT-LPNs showed uniform spherical shape with an average particle size of 127.0±2.6 nm. Rats in the model group showed strong SDL, significantly decreased blood flow in the vestibular nucleus, and abnormal levels of LAC, MDA, LDH and SOD in the hippocampus (P < 0.05 or P < 0.01). Bai-TAT-LPNs treatment significantly shortened SDL (P<0.01), increased blood flow in vestibular nucleus tissue (P<0.01), decreased the contents of LAC, MDA and LDH in hippocampus, and increased SOD content (P < 0.05 or P < 0.01). These effects of Bai-TAT-LPNs were better than the Bai group. The expression of BDNF protein in Bai group and Bai-TAT-LPNs group was significantly higher than that in sham operation group, and the increase was more obvious in Bai-TAT-LPNs group (P<0.01). Conclusions Bai-TAT-LPNs have a good Preventive effect on cerebral ischemic vertigo rats, which may be related to increasing vestibular nuclear blood flow, reducing oxidative damage and up-regulating the expression of BDNF protein in brain hippocampus. |
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