| 於建平.慢性阻塞性肺疾病患者外周血单个核细胞CD36 mRNA、血清ApoE水平对急性加重期发生的预测价值[J].浙江中西医结合杂志,2023,33(9): |
| 慢性阻塞性肺疾病患者外周血单个核细胞CD36 mRNA、血清ApoE水平对急性加重期发生的预测价值 |
| Predictive value of CD36 mRNA in peripheral blood mononuclear cells and serum ApoE levels in patients with chronic obstructive pulmonary disease in acute exacerbation |
| 投稿时间：2022-08-03 修订日期：2023-01-15 |
| DOI： |
| 中文关键词: 慢性阻塞性肺疾病 白细胞分化抗原36 载脂蛋白E 急性加重期 |
| 英文关键词:chronic obstructive pulmonary disease cluster of differentiation 36 apolipoprotein E acute exacerbation |
| 基金项目: |
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| 中文摘要: |
| 目的：分析慢性阻塞性肺疾病（COPD）患者外周血单个核细胞（PBMCs）白细胞分化抗原36（CD36）信使核糖核酸（mRNA）、血清载脂蛋白E（ApoE）水平对急性加重期发生的预测价值。方法：选取2021年6月-2022年5月在湖州市中心医院就诊的COPD患者96例，其中急性加重期COPD患者为AECOPD组（n=50），稳定期COPD患者为稳定组（n=46），选取健康人群40例为对照组。检测所有受试者血清炎性因子[肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-6、IL-1β、C反应蛋白（CRP）]、ApoE、肺功能指标[第1秒用力呼气容积与用力肺活量的比值（FEV1/FVC）、第1秒用力呼气容积占预计值百分比（FEV1%pred）]和PBMCs中CD36 mRNA水平；分析COPD患者PBMCs中CD36 mRNA、血清ApoE水平与炎性因子、肺功能指标的相关性以及PBMCs中CD36 mRNA、血清ApoE对COPD患者急性加重期发生的预测价值。结果：对照组、稳定组、AECOPD组吸烟史比例、TNF-α、IL-6、IL-1β、CRP、PBMCs中CD36 mRNA和血清ApoE水平依次升高，FEV1/FVC、FEV1%pred水平依次降低（P＜0.05）；COPD患者PBMCs中CD36 mRNA与血清ApoE水平呈正相关（P＜0.05），COPD患者PBMCs中CD36 mRNA、血清ApoE水平均与血清TNF-α、IL-6、IL-1β、CRP呈正相关（P＜0.05），与FEV1/FVC、FEV1%pred呈负相关（P＜0.05）；PBMCs中CD36 mRNA单独、血清ApoE单独、二者联合预测COPD患者急性加重期发生的曲线下面积（AUC）分别为0.887、0.871、0.966，二者联合预测的AUC高于CD36 mRNA、ApoE单独预测的AUC（P＜0.05）。结论：COPD患者PBMCs中CD36 mRNA和血清ApoE均呈高表达，二者对COPD患者急性加重期发生具有一定的预测价值。 |
| 英文摘要: |
| Objective: To analyze the predictive value of cluster of differentiation 36 (CD36) messenger RNA (mRNA) of peripheral blood mononuclear cells (PBMCs) and serum apolipoprotein E (ApoE) levels in patients with chronic obstructive pulmonary disease (COPD) for the occurrence of acute exacerbation. Methods: Ninety-six patients who came to Huzhou Central Hospital from June 2021 to May 2022 and were diagnosed with COPD were selected. Among them, patients with acute exacerbation COPD were in the AECOPD group (n=50), and patients with stable COPD were in stable group (n=46), 40 healthy people were selected as the control group. Serum inflammatory factors[tumor necrosis factor-α (TNF-α), interleukin (IL)-6, IL-1β, C-reactive protein (CRP)], ApoE, lung function indicators[ratio of forced expiratory volume in the first second to forced vital capacity (FEV1/FVC), percentage of the first second forced expiratory volume in expected value (FEV1%pred)] and CD36 mRNA levels in PBMCs were detected in all subjects; analyze the correlation between CD36 mRNA in PBMCs and serum ApoE levels of COPD patients and inflammatory factors, lung function indicators, and the predictive value of CD36 mRNA in PBMCs and serum ApoE for the occurrence of acute exacerbation in COPD patients. Results: The proportion of smoking history, TNF-α, IL-6, IL-1β, CRP, CD36 mRNA in PBMCs and serum ApoE levels in the control group, stable group and AECOPD group were increased in turn , and the levels of fev1/fvc and FEV1% PRED decreased in turn, FEV1/FVC, FEV1%pred levels were decreased in turn (P<0.05); there was a positive correlation between CD36 mRNA in PBMCs and serum ApoE level in COPD patients (P<0.05), CD36 mRNA in PBMCs and serum ApoE levels of COPD patients were correlated with serum TNF-α, IL-6, IL-1β, CRP was positively correlated (P<0.05), and negatively correlated with FEV1/FVC, FEV1%pred (P<0.05); the area under curve (AUC) of CD36 mRNA in PBMCs alone, serum ApoE alone and their combination predicting the occurrence of acute exacerbation in COPD patients were 0.887, 0.871 and 0.966, respectively, the AUC predicted by both of them is higher than that predicted by CD36 mRNA and ApoE alone (P<0.05). Conclusion: CD36 mRNA in PBMCs and serum ApoE are highly expressed of COPD patients, Both of them have certain predictive value for the occurrence of acute exacerbation in COPD patients. |
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