| 张存明,王军卫,陈松,吴忠标,叶海波.血管活性肠多肽对老龄勃起功能障碍大鼠的作用及机制研究[J].浙江中西医结合杂志,2023,33(6): |
| 血管活性肠多肽对老龄勃起功能障碍大鼠的作用及机制研究 |
| Effects of mechanism of Vasoactive Intestinal Polypeptide on Aged Rats with Erectile Dysfunction |
| 投稿时间：2022-08-13 修订日期：2023-05-04 |
| DOI： |
| 中文关键词: 血管活性肠多肽 环磷酸腺苷 蛋白激酶A 勃起功能障碍 衰老 |
| 英文关键词:vasoactive intestinal polypeptide cyclic adenosine monophosphate protein kinase A erectile dysfunction aging |
| 基金项目:温岭市科技计划项目(2018C310012) |
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| 中文摘要: |
| 目的 观察血管活性肠多肽对老龄勃起功能障碍(ED)大鼠模型中的作用及其机制。方法 通过饲养18月构建老龄大鼠,阿扑吗啡实验筛选老龄ED模型大鼠。将老龄ED大鼠分为正常组、模型组、实验组,每组7只。正常组大鼠为老龄勃起功能正常大鼠；模型组为老龄ED大鼠；实验组为老龄ED大鼠使用血管活性肠多肽25ng/kg隔日腹腔注射,治疗28天。阴茎海绵体内压(ICP)及平均动脉压(MAP)评估勃起功能；酶联免疫吸附法检测cAMP、NO含量；组织免疫荧光技术(IF)测血管性血友病因子(vWF)在海绵体组织中的表达水平；蛋白质印迹法检测海绵体蛋白激酶A(PKA)、内皮源性一氧化氮合酶(eNOS)、血管性血友病因子(vWF)、血管内皮生长因子(VEGF)蛋白表达水平。结果 正常组、模型组和实验组基础 ICP分别为(20.41 ± 5.92),(21.76 ± 5.37),(18.54 ± 3.97)mmHg,MAP分别为(123.5 ± 14.74),(118.8 ± 10.97),(114.2 ± 12.21)mmHg,各组间无统计学差异。与正常组相比,模型组Max ICP与Max ICP/MAP[Max ICP: (42.10 ± 6.57)mmHg比(94.82 ± 9.71)mmHg；Max ICP/MAP：0.36 ± 0.08比0.78 ± 0.16,P<0.01]相比明显降低。与模型组相比,实验组Max ICP与Max ICP/MAP[Max ICP: (59.52 ± 2.20)mmHg比(42.10 ± 6.57)mmHg；Max ICP/MAP：0.52 ± 0.06比0.36 ± 0.08,P<0.05]相比明显升高。模型组血管性血友病因子(vWF)荧光强度明显减少,实验组明显增加。cAMP含量分别为(94.63 ± 9.36),(33.11 ± 11.82),(58.46 ± 9.48)pmol/mg,NO含量分别为(11.59 ± 2.43),(3.05 ± 1.51),(5.31 ± 1.39)nmol/mg,PKA蛋白表达分别为1.04 ± 0.22、0.66 ± 0.08、0.97 ± 0.11,eNOS蛋白表达分别为0.71 ± 0.09、0.54 ± 0.13、0.68 ± 0.10,vWF蛋白表达分别为2.04 ± 0.24、1.22 ± 0.30、1.82 ± 0.39,VEGF蛋白表达分别为0.82 ± 0.18、0.45 ± 0.13、0.65 ± 0.07。模型组与正常组相比,实验组与模型组相比,差异均具有统计学意义(P < 0.05或P < 0.01)。结论 血管活性肠多肽可能通过调控cAMP/PKA信号通路与海绵体内皮细胞功能从而改善老龄大鼠勃起功能障碍。 |
| 英文摘要: |
| To observe the effect of vasoactive intestinal polypeptide on the aging rat model of erectile dysfunction and its possible mechanism. Methods Aged rats were constructed by feeding for 18 months, and aged erectile dysfunction (ED) model rats were screened by apomorphine experiment. The aged ED rats were divided into normal group, model group and experimental group, with 7 rats in each group. The normal group of rats were aged rats with normal erectile function; the model group was aged ED rats; the experimental group was aged ED rats received intraperitoneal injection of 25ng/kg of vasoactive intestinal polypeptide every other day for 28 days. Intracavernosal pressure (ICP) and mean arterial pressure to assess erectile function; enzyme-linked immunosorbent assay to detect cAMP and NO content; tissue immunofluorescence technique (IF) to detect the expression level of vWF in corpora cavernosa tissue; western blot to detect corpus cavernosa Protein kinase A (PKA), endothelial nitric oxide synthase (eNOS), von Willebrand factor (vWF), vascular endothelial growth factor (VEGF) protein expression levels. Results The basal ICP was (20.41?5.92), (21.76?5.37), (18.54?3.97) mmHg and MAP was (123.5?14.74), (118.8?10.97), (114.2?12.21) mmHg in the normal, model and experimental groups respectively. (118.8?10.97), (114.2?12.21) mmHg, respectively, with no statistical difference between the groups. Compared to the normal group, Max ICP and Max ICP/MAP in the model group [Max ICP: (42.10?6.57) mmHg vs. (94.82?9.71) mmHg; Max ICP/MAP: 0.36?0.08 vs. 0.78?0.16, P?0.01] was significantly lower compared to the model group. Compared with the model group, the experimental group Max ICP vs Max ICP/MAP [Max ICP: (59.52?2.20) mmHg vs (42.10?6.57) mmHg; Max ICP/MAP: 0.52?0.06 vs 0.36?0.08, P?0.05] was significantly higher compared to The fluorescence intensity of vascular haemophilic factor (vWF) was significantly reduced in the model group and significantly increased in the experimental group. cAMP levels were (94.63?9.36), (33.11?11.82), (58.46?9.48) pmol/mg, respectively, and NO levels (11.59?2.43), (3.05?1.51), (5.31?1.39) nmol/mg, respectively, and PKA protein expression was 1.04?0.22, 0.66?0.08, 0.97?0.11, eNOS protein expression was 0.71?0.09, 0.54?0.13, 0.68?0.10, and vWF protein expression was 2.04?0.24, 1.22?0.30, 1.82?0.39, and VEGF protein expression was 0.82?0.18, 0.45?0.13, 0.65?0.07, respectively. The differences were statistically significant in the model group compared with the normal group and in the experimental group compared with the model group (P?0.05 or P?0.01). Conclusion The vasoactive intestinal polypeptide may improve the endothelial cells of the cavernous endothelial cells through the cAMP/PKA signaling pathway to improve erectile dysfunction in aged rats. |
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