| 钟家浩,姚文栋,谢先泽,徐恒武.不同部位载药可溶性微针载药量比较及体外评价[J].浙江中西医结合杂志,2023,33(6): |
| 不同部位载药可溶性微针载药量比较及体外评价 |
| Drug loading comparison and in vitro evaluation of different position drug-loaded dissolving microneedles |
| 投稿时间：2022-09-23 修订日期：2022-12-20 |
| DOI： |
| 中文关键词: 可溶性微针 载药部位 载药量 体外评价 |
| 英文关键词:dissolving microneedle drug loading capacity drug-loading position in vitro evaluation |
| 基金项目:浙江省药学会医院药学专项科研资助项目（石药专项）（2019ZYY23）, |
|
| 摘要点击次数: 510 |
| 全文下载次数: 2 |
| 中文摘要: |
| 目的 制备不同部位载药可溶性微针（DMN），探究其载药量并对其进行体外评价。 方法 通过两步离心法制备不同部位载药的DMN。显微镜观察DMN形态特征。然后，通过HF-50数显推拉力计测定不同部位载药DMN机械强度；HPLC测定不同部位载药的DMN载药量；Franz透皮扩散池考察不同部位载药的DMN体外透皮性能及释放趋势。 结果 所制备的三种不同部位载药DMN均为金字塔形。不同部位载药的三种DMN机械强度无显著性差异。相较于针体载药的DMN（DMN-A），背衬层载药的DMN（DMN-B）、针体与背衬层均载药的DMN（DMN-C）载药量均显著增加，分别为DMN-A的5.75、6.83倍。DMN-A透皮释放速率最快，在4 h基本释放完全；DMN-B释放速率在三者中最慢；而DMN-C释放速度介于两者之间，释放速率接近DMN-B。 结论 本研究通过对三种不同部位载药的微针评价，增加载药空间明显提高了载药量；后续设计可以改进靠近微针的前部背衬层用于载药，增加载药空间的同时避免药物的浪费。 |
| 英文摘要: |
| Objective Preparing different positions drug-loaded dissolving microneedles (DMN) to explore their drug loading capacity and evaluate in vitro. Methods Different position drug-loaded dissolving microneedles were prepared by two-step centrifugation. The morphology of DMN were observed by microscope. Then, the mechanical strength of DMN were measured by HF-50 digital push-pull meter; the drug loading capacity of different positions drug-loaded DMN were determined by HPLC; the in vitro transdermal performance and release of drug-loaded DMN were investigated by Franz transdermal diffusion cell. Results The three types DMN were all pyramid-shaped. There was no significant difference in the mechanical strength of the three DMN. Compared with the drug-loaded DMN in the needle body (DMN-A), the drug-loaded DMN in the backing layer (DMN-B)、the DMN in both the needle body and the backing layer (DMN-C) were significantly increased in drug loading capacity, which were 5.75 and 6.83 times that of DMN-A, respectively. The transdermal release of DMN-A was the fastest, and the release rate was basically complete in 4 h; the release rate of DMN-B was the slowest among the three DMN; the release rate of DMN-C was between the DMN-A and DMN-B, and the release rate was close to that of DMN-B. Conclusion In this study, through the evaluation of the DMN loaded with drugs in three different positions, the drug loading capacity was improved significantly by increasing the drug loading space; Subsequent designs could add the front backing layer near the needle bodies for drug loading, increasing the drug loading space and avoiding drug waste. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
