| 王密,翁春燕.血根碱通过抑制PI3K/AKT通路抑制结肠癌的机制研究[J].浙江中西医结合杂志,2023,33(9): |
| 血根碱通过抑制PI3K/AKT通路抑制结肠癌的机制研究 |
| Sanguinarine inhibits colon cancer by suppressing PI3K/AKT signaling pathway |
| 投稿时间：2022-12-07 修订日期：2023-08-19 |
| DOI： |
| 中文关键词: 关键词 血根碱 结肠肿瘤 增殖 迁移。 |
| 英文关键词:Sanguinarine colon cancer proliferation migration. |
| 基金项目: |
|
| 摘要点击次数: 586 |
| 全文下载次数: 10 |
| 中文摘要: |
| 目的 研究血根碱（sanguinarine，San）在大肠癌细胞增殖和迁移中的作用及其作用机理。
方法 目的探讨不同剂量 San (2,4,8μ?mol/L）对大肠癌细胞系（HCT8、SW620）进行治疗，并应用细胞计数仪CCK-8及细胞克隆试验观察各组细胞的生长情况；用蛋白印迹的方法测定了相关蛋白的表达；建立HCT8皮下瘤体模型，采用10?mg/kg的 San液进行腹腔内灌注，以探讨其对结肠癌的抑制作用。
结果 CCK-8和细胞系的形成试验表明，San对大肠癌的增殖和增殖有一定的影响；细胞划痕试验表明，San会显著抑制大肠癌细胞的转移；免疫印迹试验表明，San能显著降低大肠癌组织中N-cadherin、PI3K、p-AKT和?mTOR的表达，使E-cadherin蛋白的表达增加；体内试验表明，San液对皮下肿瘤的生长有显著的抑制作用。
结论 血根碱能抑制大肠癌细胞的增殖和迁移，具有一定的体内外抑瘤活性，并且能调节结肠癌细胞的PI3K/AKT信号通路。 |
| 英文摘要: |
| Objective: To explore the inhibited function and possibly mechanism of sanguinarine (San) to colon cancer. Methods: Colon cancer cell lines (HCT8 and SW620) were treated with different concentrations of San (2, 4, 8μmol/L). Cell proliferation was detected by cell counting kit (CCK-8) and cell clone formation, and cell migration was detected by wound-healing assay. The expressions of related proteins were detected by Western blot. HCT8 subcutaneous tumor mouse model was constructed and San solution was injected intraperitoneally at a dose of 10mg/kg to observe the inhibitory ability of San on subcutaneous tumor. Results: Cck-8 and clone formation assay showed that San had a certain effect on the proliferation and proliferation of colorectal cancer. The cell wound-healing assay indicated that hat San could significantly inhibit the metastasis of colorectal cancer cells. Western blot showed that San significantly decreased the expression of N-cadherin, PI3K, p-Akt and mTOR, and increased the expression of E-cadherin protein in colorectal cancer. In vivo, In vivo, it has been found that San solution had a significant inhibitory effect on the growth of subcutaneous tumors. Conclusion: Sanguinarine can inhibit the proliferation and migration of colorectal cancer cells, and has certain antitumor activity in vitro and in vivo, thus showing anti-tumor effects, and this effect is related to its regulation of PI3K/AKT. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
