| 秦勇,王超君,徐胜前.人参皂苷RG1上调miR-298抑制胰腺癌肿瘤生长[J].浙江中西医结合杂志,2023,33(8): |
| 人参皂苷RG1上调miR-298抑制胰腺癌肿瘤生长 |
| Ginsenoside RG1 upregulates miR-298 and inhibits tumor growth in pancreatic cancer |
| 投稿时间：2023-03-08 修订日期：2023-07-31 |
| DOI： |
| 中文关键词: 胰腺癌 RG1 miR-298 肿瘤生长 |
| 英文关键词:pancreatic cancer RG1 miR-298 tumorigenesis |
| 基金项目:浙江省基础公益研究计划项目(LGF22H160059)作者单位丽水市人民医院肝胆胰外科(秦勇,徐胜前),超声科(王超君)(丽水323000)通讯作者秦勇,Tel：18957090555；E-mailyysygd2001@163.com |
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| 中文摘要: |
| 目的分析人参皂苷RG1对胰腺癌的影响及调控机制。方法采用MTT检测0,2.5,5,10和20μM RG1对PANC-1细胞活力的影响,流式细胞术检测RG1对PANC-1细胞凋亡的影响,Western blotting检测RG1对PANC-1细胞BAX和BCL2表达的影响,Transwell实验检测RG1对PANC-1细胞侵袭能力的影响,划痕实验检测RG1对PANC-1细胞迁移能力的影响,qRT-PCR检测RG1对PANC-1细胞miR-298水平的影响；将胰腺癌细胞分为3组：对照组、RG1处理组和RG1+miR-298 inhibitor组,MTT检测细胞活力,流式细胞术检测细胞凋亡；Transwell检测细胞侵袭能力,划痕实验检测细胞迁移能力,Western blotting检测BAX和BCL2蛋白的表达；构建胰腺癌荷瘤小鼠,进行RG1处理,观察瘤体组织大小和重量改变,Western blotting检测瘤体组织BAX和BCL2蛋白的表达,qRT-PCR检测瘤体组织miR-298水平。结果RG1处理的胰腺癌PANC-1细胞活力下降,细胞凋亡比例升高,细胞迁移能力下降,细胞迁移能力下降,miR-298水平升高；miR-298抑制剂可抑制RG1处理的细胞增殖,侵袭和迁移,促进细胞凋亡,下调细胞BAX蛋白表达,上调BCL2蛋白表达。RG1处理的胰腺癌荷瘤小鼠瘤体生长减缓。结论RG1通过上调miR-298抑制胰腺癌细胞增殖,侵袭和迁移,促进细胞凋亡,在胰腺癌中发挥抑癌作用。 |
| 英文摘要: |
| Objective To analyze the effect of RG1 on the pancreatic cancer and its regulatory mechanism. Methods The effects of RG1 on the viability of PANC-1 cells were detected by MTT. The effects of RG1 on the apoptosis of PANC-1 cells were detected by Flow cytometry The effects of RG1 on the expression of BAX and BCL2 in PANC-1 cells were detected by by Western blotting. The effects of RG1 on the invasion ability of PANC-1 cells were detected by Transwell test. The effects of RG1 on the migration ability of PANC-1 cells were detected by scratch test. Pancreatic cancer cells were divided into 3groups: control group, RG1 treatment group and RG1+miR-298 inhibitor group. Cell viability was detected by MTT, and apoptosis was detected by flow cytometry; Transwell was used to detect the invasion ability of cells, scratch test was used to detect the migration ability of cells, Western blotting was used to detect the expression of BAX and BCL2 proteins; Pancreatic cancer bearing mice were constructed and treated with RG1 to observe the size and weight changes of tumor tissue. Western blotting was used to detect the expression of BAX and BCL2 proteins in tumor tissue, and qRT PCR was used to detect the level of miR-298 in tumor tissue. Results The activity of pancreatic cancer PANC-1 cells treated with RG1 decreased, the percentage of apoptosis increased, the cell migration decreased, the cell migration decreased, and the level of miR-298 increased; MiR-298 inhibitor can inhibit the proliferation, invasion and migration of RG1 treated cells, promote cell apoptosis, down regulate the expression of BAX protein and up regulate the expression of BCL2 protein. The growth of tumor in RG1 treated pancreatic cancer bearing mice slowed down. Conclusion RG1 can inhibit the proliferation, invasion and migration of pancreatic cancer cells by up regulating miR-298, promote cell apoptosis, and play an anti-tumor role in pancreatic cancer. |
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